兔血管平滑肌细胞和聚羟基丁酯(PHB)的细胞相容性实验研究  

Experimental studies on cytocompatibility of rabbit vascular smooth muscle cells and polyhydroxybutyrate

作　　者：袁文周[1] 艾玉峰[1] 熊猛[1] 夏文森[1] 张琳西[2] 董兆麟[3] 杨清芳[4] 

机构地区：[1]第四军医大学西京医院整形外科中心 [2]第四军医大学西京医院整形外科中心陕西省西安市710032 [3]西北大学 [4]西北工业大学

出　　处：《中国美容医学》2003年第1期7-9,共3页Chinese Journal of Aesthetic Medicine

基　　金：国家自然科学基金资助项目(30070777)

摘　　要：目的:探索血管平滑肌细胞和新型可降解材料聚羟基丁酯(PHB)的细胞相容性,为组织工程血管的构建寻找理想的支架材料。方法:将组织块法体外培养的兔血管平滑肌细胞种植在PHB膜片和PHB三维微孔支架上,在相差显微镜下观察细胞的粘附和生长情况,用MTT法测定细胞粘附率和细胞增殖指数,复合培养7天后进行扫描电镜观察并用流式细胞仪(FCM)测定细胞周期、DNA指数。结果:兔血管平滑肌细胞在PHB膜片上粘附率为77％,细胞增殖符合细胞的生长曲线,在PHB三维微孔支架上生长情况良好,并被证实为二倍体细胞。结论:兔血管平滑肌细胞和聚羟基丁酯(PHB)的细胞相容性较好,但细胞与材料间的粘附有待进一步改善。Objective: To study the cytocompatibility of polyhydroxybutyrate (PHB) combined with vascular smooth muscle cells (VSMCs)in vessel tissue engineering. Methods: VSMCs were cultured with PHB, then compared with PGLA and control, the morphological characters were observed with SEM, cell adhere rate and cell proliferation were detected with MTT assay, and cell cycle was detected with flow cytometer (FCM). Results: The adhere rate of VSMCs which were seeded on PHB was 77%, and the cells seeded on PHB grew well and were confirmed as diploid cells with FCM. Conclusion : The results show that PHB has a good cytocompatibility with VSMCs, but the adhere degree between cell and material should be further improved.

关 键 词：血管平滑肌细胞 聚羟基丁酯 细胞相容性 组织工程 

分 类 号：Q813.1[生物学—生物工程]