以异种脱蛋白型松质骨为支架构建组织工程骨修复骨缺损实验研究  

Research on defects repair with tissue engineering bone by scaffolds of hetergenous deproteinnizatlon bone

作　　者：陈文斌[1] 章庆国[1] 宋萍[2] 

机构地区：[1]东南大学附属中大医院整形外科,江苏省南京市210009 [2]东南大学基础医学院

出　　处：《中国美容医学》2003年第1期22-24,共3页Chinese Journal of Aesthetic Medicine

摘　　要：目的:探讨以异种脱蛋白型松质骨为支架接种成骨细胞悬液构建的组织工程骨对骨缺损修复的能力。方法:以28天胎兔颅骨为骨源,用酶消化法分离成骨细胞,在体外培养、增殖后,取第三代成骨细胞制成细胞悬液,以5 × 106mL-1浓度接种于异种脱蛋白型松质骨中,体外培养一周,然后用此复合物修复兔颅骨缺损1,5cm × 1.5cm,本组作为实验组(共10只)。对照组I(共10只),仅用异种脱蛋白型松质骨修复兔颅骨缺损。对照组Ⅱ(共10只),缺损区注射相同浓度的成骨细胞悬液。对照组Ⅲ(共10只)为空白对照。术后4、8周从X片上观察对X-Ray阻射情况。8周取材,进行大体标本、扫描电镜及组织学评价。结果:实验组:4、8周X摄片均显示骨缺损周围有骨小梁通过,支架上有钙化阴影并随时间延长密度增高。术后8周,大体标本观察:缺损周围与正常骨组织之间无分界线,植入物硬度接近于正常骨组织,扫描电镜观察:支架上有胶原纤维及成骨细胞附着,组织学分析:支架上有大量新骨形成,支架有部分被降解吸收,四环素荧光标记证实在支架上有新生骨。所有对照组均无新生骨,仅见缺损区有纤维样组织覆盖。结论:以异种脱蛋白型松质骨为支架构建的组织工程骨可以用来修复骨缺损。Objective: To study the ability of tissue engineering bone estabilished by seeding osteoblasts into scaffolds of porous heterogenous deproteinization bone( DPB ) to repair rabbit cranial defects. Methods: The osteoblast was acquired form the cranial bone of rabbit of fetal 28 days old after digesting by enzyme, they were cultured and proliferated in vitro, the third generation of those osteoblasts was seeded into the porous heterogenous DPB with three dimensions stucture at the density of 5 ×106 mL-1, those compound was co - cultured for one week and then used to repair the defect with the area of 1. 5cm × 1. 5cm and deepth to the dural on the rabbit, ten rabbits included in the experiment group. The same defect was then served as control receiving heterogenous porous DPB, osteoblasts, and nothing respectively in the three control groups, each group included ten rabbits. In the following 4, 8 weeks after the plantation respectively, the results were assessed by x - ray, and at 8 weeks by gross inspection, scanning electron microscopy(SEM), and histology examination respectively. Results: At 4, 8 weeks, experimental group: bone trabecula was observed to pass the defect by x - ray, and the calicified shadow was observed on the scaffod. At 8 weeks, gross inspection: no demarcation between the region of the bone defect and the normal bone, the xenograft with the consistency similar to the normal bone. SEM examination: collagen fibers and osteoblasts adhered to the scaffolds . Histology analysis: plenty of new bone formed on the scaffolds. Part of those scaffolds was degraded, and the formation of new bone was then confirmed by the tetracycline fluorescence labelling method. In control groups only fibrous tissue was observed in the defect region, but no new bone formation. Conclusion: Tissue engineering bone composed with scaffods of heterogenous DPB and osteoblasts could be used for the repair of bone defect

关 键 词：实验研究 组织工程 脱蛋白型松质骨 成骨细胞 

分 类 号：R681[医药卫生—骨科学]