卡托普利抗小鼠维甲酸性骨质疏松的作用探讨  

A study on Captopril antagonized osteoporosis induced by retinoic acid in mice and it's dose-response effects

作　　者：陈方[1] 吴铁[1] 崔燎[1] 张少芬[2] 

机构地区：[1]广东医学院药理学教研室,湛江524023 [2]广东省湛江市第二人民医院检验科,湛江524003

出　　处：《中国药理学通报》2002年第5期572-575,共4页Chinese Pharmacological Bulletin

基　　金：广东省科技计划重点项目资助 NoA3 0 10 3 0 2 0 1

摘　　要：目的　观察不同剂量卡托普利对抗小鼠维甲酸性骨质疏松的量效关系 ,探讨卡托普利抗骨质疏松的骨代谢作用特点及骨药理学作用机制。方法　用维甲酸 (10 5mg·kg-1)造成小鼠骨丢失为主的骨质疏松模型 4组 ,同时其中 3组再用 3种剂量卡托普利分别给药 ,实验过程监测体重 ,2wk后处死取肝、脾、胸腺称重 ,并取股骨进行骨干重、骨钙、磷、镁、锌、锰、硫及骨羟脯氨酸 (B HOP)的测定和比较。结果　维甲酸可造成小鼠骨丢失为主的骨质疏松 ,卡托普利可对抗维甲酸所致的小鼠骨横短径、骨基质的主要成分骨羟脯氨酸及骨矿物质的减少。结论　卡托普利可对抗维甲酸引起小鼠以骨钙、磷、镁和骨羟脯氨酸丢失为主的骨质疏松 ,其中尤以低剂量卡托普利 (6 2 5mg·kg-1)AIM To study the dose effects of captopril on antagonizing osteoporosis induced by retinoic acid and to analysis the metabolic characteristics of bone. METHODS Forty male rats were administrated by retinoic acid(105 mg·kg -1 ), in the same time, three dosages of captopril were given to thirty male rats of the forty for 2 weeks, while the body weight were measured once a week. At end point, bone weight, bone hydroxyproline, calcium, phosphor, magnesium, zinc, manganese and sulphonium of femora femora in the mice were measured. RESULTS Compared with normal control mice, retinoic acid induced osteoporosis with the lessening of bone mass, captopril antagonized the decreases of bone transverse diameter, bone hydroxyproline content and bone mineral of the mice induced by retinoic acid. CONCLUSION Captopril antagonized the osteoporosis induced by retinoic acid in mice with the lessening of bone calcium, phosphor, magnesium and bone hydroxyproline. The low dose of captopril (6 25 mg·kg -1 )indicated the action remarkably.

关 键 词：卡托普利 骨羟脯氨酸 维甲酸性骨质疏松 药理学 骨代谢 动物实验 抗骨质疏松药物 

分 类 号：R681[医药卫生—骨科学] R982[医药卫生—外科学]