中国北方5城市慢性乙型肝炎患者中SEN病毒检测  

Detection of SEN virus in sera of patients with chronic hepatitis B and general population in 5 cities of China

作　　者：闫杰[1] 何忠平[1] 庄辉[2] 董庆鸣[1] 宋淑静[1] 朱林[3] 王小红[2] 

机构地区：[1]北京地坛医院病毒研究室,100011 [2]北京大学医学部病原生物学系 [3]北京市东城区疾病预防控制中心流行病科

出　　处：《中华流行病学杂志》2003年第1期33-35,共3页Chinese Journal of Epidemiology

基　　金：北京市卫生局肝炎重点学科资助

摘　　要：目的　了解SEN病毒D和H(SENV D H)在中国慢性乙型肝炎患者 (CHB)中的感染情况。方法　应用巢式聚合酶链反应法 (nPCR) ,对中国北方 5城市 5 95例CHB患者和北京市 96名正常人血清检测SENV D HDNA ,并用PCR产物直接测序法对 7株SENV进行了序列分析。结果CHB患者SENV感染率为 61.3 % ,正常人为 62 .5 % ,两者差异无显著性 ( χ2 =0 .0 47,P =0 .82 9)。但不同城市CHB患者中SENV感染率存在一定差异。从不同城市分离的SENV的核苷酸序列同源性较高 ,4株SENV D同源性为 91%～ 98% ,3株SENV H同源性为 95 %～ 98%。结论　在 5城市CHB患者中存在SENV D H感染 ,其感染率与正常人群相似 ,提示SENV DObjective To study the prevalence of SEN virus (SENV) infection in CHB patients in five cities of China. Methods A nest-polymerase chain reaction (nPCR) was used for detection of SENV-D and SENV-H in sera of 595 CHB patients from 5 cities of China and 96 normal individuals from Beijing. A total of 7 SENV strains were analyzed by direct sequencing. Results The prevalence rates of SENV in CHB patients and normal individuals were 61.3% and 62.5%, respectively ( χ 2= 0.047, P= 0.829). The prevalence rates of CHB patients between 5 cities were different. Nucleotide sequence analysis showed that the homology between 4 SENV-D strains was 91%-98% and 95%-98% between 3 SENV-H strains isolated from 5 cities in China. Conclusion SENV-D/H were prevalent in CHB patients of China and their prevalence rates were similar to that in normal individuals.

关 键 词：慢性乙型肝炎 SEN病毒 巢式聚合酶反应 CHB 

分 类 号：R512.62[医药卫生—内科学]