γ-氨基丁酸对抗精子抗体阳性患者精子顶体反应的影响及机制探讨  

Effect of GABA on the sperm acrosome reaction in antisperm antibody positive patients

作　　者：边淑玲[1] 张玮[1] 朱辉[1] 倪江[1] 姚兰春[1] 张辉[1] 陈亮[2] 

机构地区：[1]哈尔滨医科大学生理教研室,哈尔滨150086 [2]哈尔滨医科大学附属二院不育门诊

出　　处：《中国男科学杂志》2002年第5期355-357,共3页Chinese Journal of Andrology

基　　金：黑龙江科学技术厅攻关课题(GC01C142-01)

摘　　要：目的 研究γ-氨基丁酸(gamma-aminobutyric acid,GABA)对正常人类精子及抗精子抗体(AsAb)阳性精子顶体反应的影响及其机制。方法 两组各18例采用三色染色法检测精子顶体反应率。结果 GABA增加正常及AsAb阳性患者精子顶体反应率,与对照组比较,差异显著(P<0.01);GABA可使精子的Na^+—K^+—ATPase,Ca^(2+)—ATPase的活性增加(P<0.01;P<0.05);并且显著减少精子中的MDA含量(P<0.01),抑制氧自由基的产生。结论 GABA可明显提高正常人及AsAb阳性患者精子顶体反应。Objective To investigate the effect of GABA( Gamma-aminobutyric acid) on the rate of sperm acrosome reaction both in normal and positive antisperm antibody men. Methods The sperm acrosome reaction was tested with triple-stain technique, in two groups 18 cases each. Results (1) GABA could increase the rate of sperms acrosome reaction both in normal and AsAb positive patients(P<0. 01) ; (2) GABA could increaseNa+ -K+ -ATPase activity of sperms (P< 0.001); (3) GABA could increase Ca2+ - ATPase activity of sperms (F<0. 05); (4) GABA could decrease the production of MDA and oxygen free radicals of sperms. Conclusions GABA could regulate the rate of sperm acrosome reaction.

关 键 词：Γ-氨基丁酸 抗精子抗体 精子顶体反应 影响 免疫不育 

分 类 号：R698.2[医药卫生—泌尿科学] R392.[医药卫生—外科学]