双峰驼诱导排卵机理的研究.Ⅱ.双峰驼诱导排卵因子的靶器官及代谢动力学  

Study on Ovulation Inducing Mechanism in Bactrian Camel Ⅱ Target Organ of Ovulation Inducing Factor (OIF) and Its Metabolic Dynamics in Bactrian Camel

作　　者：潘光武[1] 谢庆阁[1] 才学鹏[1] 汪静[2] 邓敬兰[2] 刘湘涛[1] 潘元青[3] 陈北亨[4] 

机构地区：[1]中国农业科学院兰州兽医研究所,兰州730046 [2]第四军医大学西京医院,西安710032 [3]兰州医学院,兰州730010 [4]甘肃农业大学,兰州730070

出　　处：《中国农业科学》2003年第1期105-110,共6页Scientia Agricultura Sinica

基　　金：国家自然科学基金资助项目 (3 92 70 5 3 4;3 9670 5 3 9) ;兰州兽医研究所所长基金资助项目

摘　　要：应用同位素示踪技术 ,将公驼精清内的诱导排卵活性蛋白 (OIP)经12 5I标记后输入母驼阴道。示踪结果证明 ,OIP经阴道前端主动吸收 ,很快进入血液循环 ,其代谢动力学属于一级吸收二室开放模型 ,吸收速度常数 (ka)为 0 .0 6 7·min-1,吸收半衰期 10 .2 4min ,于 2 3min达峰浓度 (Cmax) 5 .15 μg·ml-1,清除半衰期 (t1/2 k)较长为 72 .6h ;从DEAE DE52 柱上洗脱的活性蛋白吸收后 ,不经过脊髓和卵巢反馈通路 ,而是经血液循环直达脑髅内的靶器官垂体 ,从而引起母驼释放促黄体素 (LH) ,至峰值出现 。The main site for active absorption of OIF is situated in the anterior vagina after insemination of ovulation inducing bioactive protein (OIP) from seminal plasma in male camel which was iodinated with 125 I of isotope. OIP is assimilated quickly into the blood circulation and its metabolic dynamic pattern belongs to the first order absorption and 2 compartment open model, absorbent rate constant (ka) was 0.067·min -1 , absorbent period of the half life (t 1/2 ka) was 10.24 min, calculated peak concentration 5.15μg·ml -1 appeared at 23 min after administration of OIP, Elimination of the half life period (T 1/2 k) needs a long time of 72.6 h. OIP eluted on DEAE DE 52 column is directly reached to target gland pituitary in the crania via the blood circulation after insemination and absorption instead of regulation by the cerebro spinal fluid system or ovaries feedback mechanism, Thus resulting in a preovulation peak of LH in female camel.

关 键 词：诱导排卵机理 代谢动力学 双峰驼 诱导排卵因子 靶器官 垂体 同位素示踪技术 

分 类 号：S824[农业科学—畜牧学]