SR92A系黄羽肉鸡与萧山鸡及其杂种后代的DNA指纹分析  

DNA Fingerprinting of SR92A Chicken,Xiaoshan Chicken and Their Crossbred Chicken

作　　者：刘向萍[1,2] 王生存[1] 王金玉[1] 戴国俊[1] 

机构地区：[1]扬州大学畜牧兽医学院,江苏扬州225009 [2]中国农科院家禽研究所

出　　处：《中国畜牧杂志》2003年第1期13-14,共2页Chinese Journal of Animal Science

基　　金：国家自然科学基金项目(39870566)资助

摘　　要：本文以EAV(禽内源性反转录病毒片段 )为探针 ,EcoRI为限制性内切酶 ,对萧山鸡、SR92A系及其杂交后代进行了DNA指纹图谱的研究。结果表明 :EAV探针适合于鸡的DNA指纹分析 ,能产生清晰可辩的图谱。平均条带总数为11.384± 0 .84 4 ,两品种间、公母间条带总数均无显著差异。萧山鸡和SR92A系品种内相似系数分别为 0 .5 81、0 .5 11,高于品种间相似系数 (0 .2 31)。杂交后代相似系数为 0 .30 1。DNA fingerprinting of SR92A chicken,Xiaoshan chicken and their crossbred chicken were established with EAV(endogenous avian retroviral element)as the probe.It was revealed that similarity coefficient of crossbred chicken(0.301)was much lower than both of Xiaoshan chicken(0.581)and of SR92A chicken(0.511).The average number of bands are 11.384±0.844

关 键 词：畜牧、兽医科学基础学科 DNA指纹分析 探针 鸡 

分 类 号：S831.2[农业科学—畜牧学]