两株东北红豆杉内生真菌的生理特性  

Physiological Characteristics of Two Endophytes for Taxus cuspidata

作　　者：史小凤[1] 项勇[1] 王晓燕[2] 吕安国[3] 吴文芳[3] 

机构地区：[1]沈阳药科大学制药工程学院,沈阳110016 [2]东北林业大学 [3]中国科学院应用生态研究所

出　　处：《东北林业大学学报》2003年第1期69-71,共3页Journal of Northeast Forestry University

摘　　要：采用接种法 ,对两株东北红豆杉内生真菌散囊菌 (Eurotiumsp .,10 0 1)和曲霉 (Aspergillussp .,10 0 3)在不同温度、pH、碳源、氮源、培养基及糖质量浓度下进行了生理实验研究。结果表明 :温度对两种菌株的生长和孢子的产生具有明显的影响 ;pH6～ 7时菌株生长良好 ,且可以保持较高的菌液质量浓度 ;两种菌株均以蔗糖为主要碳源 ,菌丝干质量与培养基质量浓度呈正向关系。Two endophytes Eurotium sp.1001 and Aspergillus sp.1003 isolated from Taxus cuspidata Sieb et Zucc. are the study subjects in this paper. The temperature, carbon sources, nitrogen sources, pH, the concentration of the media and saccharides and the requirement of some general vitamins as well as the relationship of the two strains are studied. The result shows that temperature has significant effect on the growth of these two endophytes and the production of spores. pH 6～7 is suitable for the growth of endophytes. The dry weight of hyphae has positive correlation with the concentration of culture medium.

关 键 词：生理特性 东北红豆杉 内生真菌 接种法 

分 类 号：S791.49[农业科学—林木遗传育种]