狨猴(Saguinus mystax)血红蛋白(Hb)α链一级结构的测定  

THE PRIMARY STRUCTURE OF HEMOGLOBIN a CHAIN OF MARMOSET (Saguinus mystax)

作　　者：杨龙春 金力[1] 柴常星[1] 刘祖洞[1] 余家璜 

机构地区：[1]复旦大学遗传学研究所 [2]上海实验动物研究中心

出　　处：《复旦学报（自然科学版）》1992年第2期200-205,共6页Journal of Fudan University：Natural Science

基　　金：国家自然科学基金

摘　　要：分析了狨猴(Saguinus mystax)血红蛋白(Hb)a链的氨基酸序列.经CM-23纤维素柱层析得到的狨猴Hb珠蛋白a链,分别用溴化氰裂解、胰蛋白酶和糜蛋白酶消化,经高效液相层析(HPLC)和高压电泳-纸层析分离后的肽段,用蛋白质顺序自动分析仪测定其氨基酸序列.结果表明,完整狨猴a链的一级结构与人、恒猴和懒猴的a链有差异,肯定了狨猴的进化地位.同时对测定一级结构的方法进行了讨论.a Chain of hemoglobin from Saguinus mystax was isolated by CM-23 cellulose column chromatography. Desalted a Chain was cleaved by cyanogen bromide, TPCK-trypsin and chymotrypsin respectively. Peptides were isolated and purified by HPLC and high voltage electrophoresis-paper chromatography. Peptide sequencing was done by Model 470 gas phase protein sequencer and DNS-Cl method. The complete amino acid sequence of hemoglobin a chain of Saguinus mystax was compared with that of human, Macaca mulatta and Nycticebus coucang. The evolutionary status of Saguinus mystax and experimental strategy of protein sequencing were discussed.

关 键 词：灵长目 血红蛋白 一级结构 狨猴 

分 类 号：Q959.848[生物学—动物学]