CTLA4Ig诱导小鼠心脏移植免疫耐受的研究  

Allograft mouse transplantation immune tolerance induced by CTLA4Ig

作　　者：胡湘明[1] 杨周灼[1] 曾仁海[2] 郑祥光[3] 

机构地区：[1]广东省人民医院肾内科,广州510080 [2]广东省人民医院病理科,广州510080 [3]广东省人民医院泌尿外科,广州510080

出　　处：《广东医学》2003年第2期140-141,共2页Guangdong Medical Journal

摘　　要：目的　研究利用CTLA4Ig阻断B7/CD2 8相结合 ,诱导同种异体小鼠移植心脏免疫耐受。方法　建立同种异体小鼠移植心脏模型 ,研究组腹腔注射CTLA4Ig ,观察小鼠移植的心脏存活天数和病理改变。结果　研究组移植的心脏存活时间为 (81 0± 5 1 2 )d ,对照组为 (7 8± 1 3 )d ,两组差异有显著性 (P <0 0 5 )。组织学改变 :对照组可见大量淋巴细胞、单核细胞浸润 ,组织充血水肿 ,心肌变性、坏死 ,出血 ,病理分级为Ⅳ级。研究组术后 7d病理改变较轻 ,表现为局灶性血管周围和心肌间质炎症细胞浸润、局灶性心肌损伤 ,病理分级为Ⅰ～Ⅱ级。术后 3 0d研究组可见部分心肌变性、水肿、纤维增生 ,炎细胞浸润 ,血管管壁增厚、水肿、炎细胞外渗 ,病理分级为Ⅱ～Ⅲ级。结论　利用CTLA4Ig阻断B7/CD2 8相结合可诱导同种异体小鼠心脏移植免疫耐受。Objective To study transplantation tolerance induced by CTLA4Ig after heart transplantation in mouse. Methods Mouse heterotopic heart allograft model was established. CTLA4Ig was administered by intraperitoneal injection at a dose of 0 2 mg. Survival duration and histological changes were measured after operation. Results Survival duration was (81 0±51 2)days in experimental group versus (7 8±1 3)days in control group. In the experimental group, the graft survival duration was significantly prolonged( P <0 05). Histological changes were characterized by massive lymphocyte infiltration, interstitial edema, myocyte injury and necrosis, and hemorrhage(grade Ⅳ) in control group. In the experimental group, only mild local lymphocyte infiltration and patchy myocyte injury(grade Ⅰ～Ⅱ) on the seventh post-operation day were found. At 30 days after operation, the pathological changes in the experimental group were also mild but showed coronary arterial intimal thickening and fibrosis consistent with chronic rejection(gradeⅡ～Ⅲ).Conclusion Mouse heterotopic heart allograft immune tolerance can be induced by CTLA4Ig.

关 键 词：CTLA4IG 小鼠 心脏移植 免疫耐受 研究 

分 类 号：R654.2[医药卫生—外科学]