基因序列分析确诊大熊猫的犬瘟热病毒感染  

Gene Sequence Analysis Diagnosis of Giant Pandas Infected by Canine Distemper Virus

作　　者：李金中[1] 夏咸柱[1] 何洪彬[1] 余春 胡贵学 范泉水 郑先春黄耕 武银莲[1] 

机构地区：[1]解放军农牧大学军事兽医研究所,吉林长春130062

出　　处：《中国兽医学报》1999年第5期448-450,共3页Chinese Journal of Veterinary Science

摘　　要：直接从死亡大熊猫肝脏提取细胞总 Ｒ Ｎ Ａ，经反转录后用犬瘟热病毒的 １ 对引物扩增出了约 ３２０ ｂｐ 的片段。此产物经纯化、序列分析表明，其片段 ２ 个引物间长度为 ２８１ ｂｐ，与预计片段大小相同。此毒株在核苷酸和氨基酸水平与北京犬等 ４ 个野毒株、哈尔滨犬野毒株、某疫苗弱毒株、 Ｏｎｄｅｒｓｔｅｐｏｏｒｔ 弱毒株和海豹瘟热病毒 ２ 型毒 株的 同源 性分 别为 ９２２％ 和 ９８９％ 、９２５％ 和 ９８９％ 、９１５％ 和 ９４６％ 、９２９％ 和 ９８９％ 、９８２％ 和 １００％ 。这样就进一步确定了大熊猫的犬瘟热病毒感染。A couple primers were applied to amplify the fusion region gene of canine distemper virus (CDV) fusion (F) protein gene. The templates were produced from the reverse transcription reaction that RNA were isolated from died giant pandas livers. About 320 bp fragments were amplified by polymerase chain reaction. The fragments were purified and sequence analysed. There were 281 base pairs in the fragments that excluded the 2 primers. The homologies with Beijing field strains, Harbin field strains, CDV vaccine attenuated strains, CDV Onderstepoort attenuated strains, phocine distemper virus type 2 (PDV 2) in nucleotide and amino acid were 92.9% and 98.9%, 92.5% and 98 9%, 91.5% and 94.6%, 92.9% and 98.9%, 98.2% and 100% respectively.

关 键 词：大熊猫 犬瘟热病毒 序列分析 PCR扩增 基因片估 

分 类 号：S858.922.6[农业科学—临床兽医学]