囊虫病循环抗原酶联免疫试剂检测盒的研制  

Development of a Enzyme linked Detecting kit of the Circulating Antigen of Cysticercus Cellulosae

作　　者：刘玉[1] 唐雨德[1] 顾志香[1] 周宗安[1] 王永山[1] 于明明[1] 郁兴民 翟春生[1] 

机构地区：[1]南京军区军事医学研究所,江苏南京210002

出　　处：《中国兽医学报》1999年第5期471-475,共5页Chinese Journal of Veterinary Science

摘　　要：以部分纯化囊虫抗原免疫 Ｂ Ａ Ｌ Ｂ／ｃ 小鼠，取其脾细胞与 Ｓ Ｐ２／０ 细胞融合制备抗囊虫循环抗原（ Ｃ Ａ） Ｍｃ Ａｂ ，共获得 ８ 株分泌抗囊虫 Ｃ Ａ Ｍ ｃ Ａｂ 细胞株。经鉴定，筛选出组合后能获得最佳检测效果的 Ｃ Ａ Ｍ ｃ Ａｂ细胞株 Ｍ ｃ Ａｂ １ Ｃ７（包被）和 Ｈ Ｒ Ｐ１ Ｂ５ 作为检测用的 Ｍ ｃ Ａｂ，用其建立了检测囊虫 Ｃ Ａ 的双抗体夹心 Ｅ Ｌ Ｉ Ｓ Ａ方法，并研制了囊虫病 Ｃ Ａ 检测试剂盒。试剂盒用于检测囊虫病 Ｃ Ａ，敏感、特异、快速、简便，囊虫病血清 Ｃ Ａ的检出率为 ９１．４３％ ，脑脊液 Ｃ Ａ 的检出率为 ９４．４４％ 。The BALB/C mice were immunized with the partly purified antigens of cysticercus cellulosae, their spleen cells and sp2/0 cells were fused. Eight hybridomas secreting monoclonal antibodies (McAbs) against the circulating antigen(CA)of cysticercns cellulosae were established. After a series of identification, the best compose of McAb 1C 7 as coating Ab and HRP 1B 5 used as detecting Ab was screened in order to obtain the best detecting results. Based on this,a sandwich ELISA was established and its detecting kit of cysticercus cellcclosae CA had been developed. The kit was sensitive, specific, quick and simple when it was used to detect cysticercus cellulosae CA. The detecting rates of sera and cerebrospinal fluid (CSF) of cysticercosis were 91 43% and 94 44%, respectively. All these arrived at most advanced levels at home and abroad.

关 键 词：囊虫病 循环抗原 酶联免疫吸附试验 试剂检测盒 

分 类 号：R532.33[医药卫生—内科学]