利用异种动物抗血清双抗夹心法检测小麦黄花叶病毒  

Detection of Wheat yellow mosaic virus by Heterogeneous Animal Double-antibody Sandwich ELISA

作　　者：耿波[1] 韩成贵[1] 翟亚锋[1] 王慧清[1] 于嘉林[1] 刘仪[1] 

机构地区：[1]中国农业大学农业生物技术国家实验室与植物病理系,北京100094

出　　处：《中国病毒学》2003年第1期76-78,共3页Virologica Sinica

基　　金：国家自然科学基金资助(39870489);"973"项目资助(G2000016201)

摘　　要：小麦黄花叶病毒(Wheat yellow mosaic virus,WYMV),属于马铃薯Y病毒科(Potyviridae),大麦黄花叶病毒属(Bymovirus),传播介体为禾谷多粘菌(Polymyxa graminis),与发生在欧美的小麦梭条花叶病毒(WSSMV)为同一属内的两种病毒[1].With purified Wheat yellow mosaic virus (WYMV) particles as antigen, cavy was injected to produce specific antiserum. Its titer was tested as 1/128 by the method of micro-immunoprecipitation. Using cavy antiserum as capture antibody for trapping the virus and rabbit antiserum as detection antibody, the experiment system of heterogeneous animal DAS-ELISA (HADAS-ELISA) was established to detect WYMV. The system has the advantage of better specificity and sensitivity of detection. This method was proved to be successful and prattical in the detection of the virus in field-collected samples , indicating that the experiment system of HADAS-ELTSA is superior to the indirect ELISA with rabbit antiserum only.

关 键 词：小麦黄花叶病毒 豚鼠抗血清 异种动物双抗夹心ELISA 

分 类 号：S432.41[农业科学—植物病理学]