牛蒡叶中微量木脂体牛蒡子甙和牛蒡子甙元的分离与鉴定  

Isolation and Identification of Trace Lignans, Arctiin and Arctigenin, in Arctium lappa L. Leaves

作　　者：刘世名[1] 陈靠山[1] SCHLEMANN Willibald SCHMIDIT Jügen STRACK Dieter 

机构地区：[1]山东大学生命科学学院,山东济南250100 [2]Institute of Plant Biochemistry

出　　处：《色谱》2003年第1期52-55,共4页Chinese Journal of Chromatography

基　　金：863"计划;山东省优秀中青年科学家奖励基金;江苏省科技厅重点项目;山东大学青年教师基金资助.

摘　　要：建立了分离鉴定牛蒡叶中微量木脂体牛蒡子甙(arctiin)和牛蒡子甙元(arctigenin)的方法。牛蒡叶粗提物经聚酰胺柱提取,浓缩其甲醇洗脱液并于低温下静置析出白色沉淀物。沉淀物用甲醇溶解后经反相高效液相色谱(RP HPLC)分离,纯化得到两个主要组分。经紫外光谱(UV)、红外光谱(FTIR)及电喷雾质谱(ESI MS)检测,并与牛蒡子甙和牛蒡子甙元对照品的UV,LC ESI MS,HPLC及FTIR图谱比较,鉴定两个主要成分为牛蒡子甙和牛蒡子甙元。The methods of polyamide column chromatography, reversedphase high performance liquid chromatography (RPHPLC), liquid chromatographyelectrospray ionization mass spectrometry (LCESIMS) and Fourier transform infrared spectroscopy (FTIR) for the isolation and identification of trace lignans, arctiin and arctigenin, in Arctium lappa L. leaves were established. When the crude extracts of Arctium lappa L. leaves were chromatographed by polyamide column, and the fraction eluted by 100% methanol were concentrated and refrigerated, a white precipitate was obtained. Two main constituents were purified from the precipitate by RPHPLC. Compared with the UV, LCESIMS, HPLC and FTIR spectra of standard samples of arctiin and arctigenin, the two main purified constituents were identified as arctiin and arctigenin. 

关 键 词：聚酰胺柱色谱 反相高效液相色谱 电喷雾质谱 红外光谱 牛蒡式甙 牛蒡子甙元 牛蒡叶 

分 类 号：O658[理学—分析化学]