某部装甲兵的精液质量分析  

ANALYSIS OF SEMEN QUALITY IN SOME TANK SOLDIERS

作　　者：丁晓萍[1] 闫素文[1] 张宁[1] 汤洁[1] 鲁海鸥[1] 唐轶[1] 王喜良[1] 柳新[1] 

机构地区：[1]解放军第二○二医院全军优生优育研究中心,沈阳110003

出　　处：《解放军预防医学杂志》2003年第1期23-26,共4页Journal of Preventive Medicine of Chinese People's Liberation Army

基　　金：全军医学科研"十五"基金重点课题 (No .0 1Z0 0 2 )

摘　　要：目的 :探讨装甲兵训练噪声振动等物理因素是否对男性精液质量产生影响 ,为制定干预措施 ,降低和预防男性不育的发生提供依据。方法 :通过填写问卷调查表、体检及采用全自动精液分析系统分析精液质量、单细胞凝胶电泳和流式细胞仪检测DNA单双链损伤以及分析染色质结构等先进实验检测方法对某部装甲兵进行分子流行病学调查。结果 :暴露组精液中精子存活率 (6 2 .30± 2 5 .2 4 ) %和正常精子比率 (81.2 8± 11.18) %较对照组 (6 8.0 2± 2 5 .72 ) %和 (93.5 6± 5 .0 3) %下降 (P <0 .0 5 ,P <0 .0 1) ,精子畸形率则明显地增高〔(9.0 6± 11.15 ) %vs (7.37±10 .6 1) % ,P <0 .0 1〕 ,但均在正常值范围内 ,仅精子活力 (36 .4 % )明显低于正常值 (>5 0 % )和对照组 (43.5 % ,P <0 .0 1)。彗星实验和染色质结构分析则无明显变化。结论 :长期受到噪声振动的影响 ,可能发生精液质量下降 ,但未发现精子DNA损伤增多。提示 ,加强装甲兵的个人防护 ,制定预防保健措施对提高男性生殖健康保健十分必要。Objective:To explore the effect of physical factors such as noise,vibration quality in tank soldiers during training, so as to provide basis for making up intervention measures to reduce and prevent male infertility. Methods: Molecular epidemiological investigation in some tank soldiers was carried out by questionnaire, physical examination and advanced lab analyses such as automatic semen analysis system to analyze semen quality, single cell gel electrophoresis and flowcytometer to detect sperm DNA damage and chromatin structure. Results: The sperm survival rate and normal sperm ratio in exposed group〔(62.30±25.24)% and (81.28±11.18)% respecitvely〕 were lower than those in control group〔(68.02±25.72)%,(93.56±5.03)% respectively, P<0.05,P<0.01〕,while sperm abberation rate in exposed group (19.06±11.15)% was higher than that in control group 〔(7.37±10.60)%,P<0.01〕. However, all these data were in normal range. The sperm vitality in exposed group(36.4%) was significantly lower than that in control(43.5%,P<0.01) and normal value(>50%). Comet assay and sperm chromatin structure assay showed no significant change. Conclusion: Long term exposure to noise and vibraton may induce decrease in semen quality but not DNA damage in sperm. It is necessary to improve individual protection and to make up preventive measures for male reproductive care in tank soldiers.

关 键 词：精液质量 装甲兵 噪声 振动 精子DNA损伤 

分 类 号：R321.1[医药卫生—人体解剖和组织胚胎学] X827[医药卫生—基础医学]