3种脾虚小鼠红细胞膜流动性变化的研究  

Study on the Change of Erythrocyte Membrane Lipid Fluidity in 3 Kinds of Mice with Spleen Deficiency Syndrome

作　　者：刘洪尊[1] 冯端浩[1] 高杉[1] 刘蔚[1] 缑宇轩[1] 孙玲[1] 史迎昌[1] 温筱煦[1] 

机构地区：[1]解放军第309医院临床药理科,北京100091

出　　处：《中国中西医结合消化杂志》2003年第1期15-17,共3页Chinese Journal of Integrated Traditional and Western Medicine on Digestion

摘　　要：目的 :研究几种脾虚证模型小鼠红细胞 (RBC)膜流动性 (L FU)的变化规律。方法 :分别用大黄、番泻叶、利血平制作脾虚模型。用血细胞分析仪检测不同模型小鼠血常规指标的变化 ,用荧光偏振法检测不同模型小鼠RBC L FU和微粘度 (η)的变化。结果 :不同造模方法对小鼠血常规指标 ,除平均血细胞容积外并无明显影响 ,但均使 3种模型小鼠 RBV L FU降低 ,η增加。结论 :RBC LObjective: To study the relationship between erythrocyte membrane lipid fluidity and the animal model of spleen deficiency syndrome. Methods: Three kinds of mouse models of spleen deficiency syndrome were induced by Radix et Rhizoma Rhei (Daihuang) infusion, Folium Sennae (Fanxieye) infusion, reserpine injection respectively. The membrane lipid fluidity of erythrocyte was measured by the fluorescence polarization technique. Results: There was obvious difference in erythrocyte membrane lipid fluidity among three kinds of mice with spleen deficiency syndrome. Conclusion: Erythrocyte membrane lipid fluidity may be considered as an objective and general index to evaluate the spleen deficiency syndrome.

关 键 词：脾虚证 动物模型 红细胞 膜流动性 

分 类 号：R256.39[医药卫生—中医内科学] R394[医药卫生—中医学]