N-硝基-L-精氨酸抗阿片类精神依赖作用  

Studies on effects of N-nitro-L-arginine against opiates psy chical dependence

作　　者：万兴旺[1] 黄矛[1] 何雅琴[2] 李万亥[3] 万莉[4] 路长林[5] 

机构地区：[1]第二军医大学基础医学部新药评价中心,上海200433 [2]第二军医大学东方肝胆外科医院国际合作生物信号转导研究中心,上海200438 [3]第二军医大学药学院药理学教研室,上海200433 [4]第二军医大学长海医院药学部,上海200433 [5]第二军医大学基础医学部神经生物学教研室,上海200433

出　　处：《中国药学杂志》2003年第1期25-28,共4页Chinese Pharmaceutical Journal

摘　　要：目的　研究N 硝基 L 精氨酸 (NO2 Arg)在吗啡、二氢埃托啡精神依赖形成中的作用。 方法　采用小鼠腹腔注射吗啡、二氢埃托啡的方法建立小鼠精神依赖的条件性位置偏爱模型 ;采用荧光内过滤法测定小鼠脑内NO的含量 ;小鼠脑内cAMP/cGMP含量采用放射免疫法测定。结果　小鼠吗啡、二氢埃托啡精神依赖形成过程中脑内NO ,cAMP ,cGMP的含量显著上升 ;腹腔注射NO2 Arg 5mg·kg- 1 可抑制吗啡、二氢埃托啡精神依赖升高小鼠脑内NO ,cAMP ,cGMP含量的作用。结论　NO在易化阿片类药物精神依赖形成中可能有一定作用 ;NO2 Arg抑制吗啡。OBJECTIVE: To investigate the role of N-nitro-L-arginine (NO2Arg) in the development of psychical dependence induced by morphine and dihydroetorphine. METHODS: The psychical dependence model was established by subcutaneous administration of morphine or dihydroetorphine into mice. The content of NO was determined by the fluorescence intensity based on the absorption of a reaction product of N-acetylcystaine and nitric oxide (NACNO) overlapping the absorption of quinine sulfate. The contents of cAMP/cGMP were measured by radioimmunoassay. RESULTS: The contents of NO, cAMP and cGMP in mice brains were increased significantly after consecutive injection of morphine and dihydroetorphine. Intra-peritoneal injection of 5 mg&middotkg-1 NO2Arg inhibited the development of psychical dependence induced by morphine and dihydroetorphine and the increases of NO, cAMP and cGMP content in brains. CONCLUSIONS: NO2Arg may suppress the development of psychical dependence induced by morphine and dihydroetorphine in mice by inhibiting the synthesis of nitric oxide and regulating the cGMP/cAMP content.

关 键 词：吗啡 二氢埃托啡 精神依赖 一氧化氮 

分 类 号：R964[医药卫生—药理学]