聪圣胶囊对拟缺血再灌注大鼠原代培养皮层神经细胞的保护作用  

Effects of Congsheng Capsule on cultured rat cerebral cortical neurons after ischemia-reperfusion.

作　　者：赵玲[1] 徐秋萍[2] 李林[1] 

机构地区：[1]首都医科大学宣武医院药理研究室,北京脑老化重点实验室,北京100053 [2]北京中医药大学药理教研室,北京100029

出　　处：《中草药》2003年第2期144-146,共3页Chinese Traditional and Herbal Drugs

基　　金："九.五"国家攻关课题 (96-90 6-0 9-0 3 )

摘　　要：目的　研究聪圣胶囊对大鼠原代培养皮层神经细胞拟缺血再灌注损伤的影响。方法　采用血清药理学和流式细胞仪的方法 ,观察聪圣胶囊含药血清对缺糖缺氧 (3h)拟缺血再灌注 (0 ,3,6 ,18h)损伤神经细胞乳酸脱氢酶 (L DH)漏出及神经细胞凋亡率的影响。结果　聪圣胶囊含药血清 (2 ,4 ,8g/ kg)可抑制缺糖 -缺氧 3h再灌 (0 ,3,6和 18h)引起的神经细胞内 L DH的释放。降低缺糖 -缺氧 3h再灌 18h导致的凋亡细胞率。结论　聪圣胶囊可减轻缺血性脑损伤。Object To investigate the profective effects of Congsheng Capsule (CSC) on cultured cerebral cortical neurons after cerebral ischemia reperfusion in order to study the anticerebral ischemia effect of CSC. Methods Drug containing serum was used to study the protective effect of CSC on neurons damaged during hypo glucos and hypoxia (3 h)/reoxygenation (0, 3, 6, 18 h), the demage to neurons was reflected by the increase in activity of lactate dehydrogenase (LDH) released from cells into culture medium and the changes of apoptosic cells which were studied by flow cytometry. Results The serum preparation of CSC (2, 4, 8 g/kg) attenuated neuronal damage during hypo glucos and hypoxia (3 h)/reoxygenation (0, 3, 6, 18 h) by decreased LDH and changes in apoptosic cell numbers. Conclusion CSC has protective effects on cerebral ischemic damage.

关 键 词：聪圣胶囊 神经细胞 脑缺血再灌注 细胞凋亡 

分 类 号：R285.5[医药卫生—中药学]