脂多糖介导的急性肺损伤大鼠血浆IL-13含量的变化  

THE CHANGES IN IL-13 CONTENT IN PLASMA IN RATS WITH ACUTE LUNG INJURY INDUCED BY LIPOPOLYSACCHARIDE

作　　者：李琦[1] 钱桂生[1] 张青[1] 张伦基 陈仲祥 

机构地区：[1]第三军医大学新桥医院全军呼吸病研究所 [2]巴中市人民医院 [3]重庆钢铁公司职工医院

出　　处：《解放军医学杂志》2003年第2期107-109,共3页Medical Journal of Chinese People's Liberation Army

基　　金：国家自然科学基金重点项目 (编号 39730 2 1 0 );全军医学科研"十五"重点项目 (编号 0 1Z0 74)资助课题

摘　　要：观察脂多糖 (LPS)介导的大鼠发生急性肺损伤 (ALI)时血浆中IL 13含量的变化 ,探讨ALI发生、发展过程中 ,抗炎因子变化的意义。由Wistar大鼠尾静脉注射梯级剂量LPS(2mg/kg、4mg/kg、6mg/kg、8mg/kg) ,复制ALI模型 ;采用酶联免疫吸附试验 (ELISA)检测血浆IL 13含量。结果发现 ,梯级剂量LPS可以引发大鼠程度不同的ALI,当LPS≥ 6mg/kg时 ,发生急性呼吸窘迫综合征 (ARDS) ;LPS可以导致大鼠血浆IL 13含量升高 ,当LPS≥ 6mg/kg时 ,血浆IL 13含量峰值显著提高。研究表明 ,尾静脉注射LPS可以复制大鼠ALI模型 ,LPS≥ 6mg/kg是大鼠发生ARDS的临界剂量 ;LPS所介导的大鼠血浆IL 13含量升高 。To investigate the interleukin 13(IL 13) content in plasma in rats with acute lung injury (ALI) induced by lipopolysaccharide (LPS), Wistar rats were given increasing doses of LPS (2mg/kg,4mg/kg,6mg/kg,8mg/kg) to produce ALI. The respirtory rate,PaO 2 ,wet weight/dry weight (W/D) of the lung, and pathological changes in the lung were observed. ELISA was used to determine plasma IL 13. It was found that: (1)ALI could be produced in rats with LPS, but ARDS occurred only when the dose of LPS reached 6mg/kg. or larger. (2)LPS produced an elevation of the content of IL 13 in plasma in rats, peaking when the dose of LPS reaching 6mg/kg or over. These results suggested that LPS might induce ALI in rats, and ARDS could be produced when the dose of LPS reached ≥6mg/kg. (3)The high increase in plasma IL 13 content might play an important role in producing ARDS induced by LPS.

关 键 词：成人型呼吸窘迫综合征 脂多糖类 白细胞介素13 

分 类 号：R563.802[医药卫生—呼吸系统]