反相高效液相色谱法同时测定胆汁中氟罗沙星、甲硝唑/替硝唑的浓度  

RP-HPLC determination of fleroxacin and flagy or tinidazol

作　　者：任志强[1] 吴为[1] 龙艾兵[1] 李宪为[1] 

机构地区：[1]益阳市中心医院,湖南益阳413000

出　　处：《中国医院药学杂志》2003年第2期80-81,共2页Chinese Journal of Hospital Pharmacy

摘　　要：目的 :建立反相高效液相色谱法 (RP HPLC)测定胆汁中氟罗沙星、甲硝唑或替硝唑浓度的方法。方法 :以乙腈 0 .0 5mol·L-1柠檬酸 (18∶82 ,三乙胺调pH为 3.8)为流动相 ,流速为 1.0mL·min-1,检测波长 2 86nm。结果 :氟罗沙星、甲硝唑、替硝唑浓度分别在 0 .388～ 7.76、0 .5 5 2～ 11.0 4、0 .4～ 8.0mg·L-1范围内线性关系良好。相关系数r分别为 0 .9999,0 .9999,0 .9998,平均相对回收率分别为 99.4 1% ,98.6 4 % ,10 0 .2 6 % ,日内和日间RSD均小于 4 .0 %。结论 :本法适用于对胆汁中OBJECTIVE To establish an RP HPLC method for the determination of fleroxacin and flagy or tinidazol in human bile. METHODS The mobile phase was acetonitrile 0.05 mol·L -1 lemon acid(18∶82 pH 3.8 adjusted by triethylamine).The flow rate was 1.0 mL·min -1 . The wavelength of UV detection was 286 nm. RESULTS The linearity was obtained in the rang of 0.388 ～ 7.76 mg·L -1 of floxacin, 0.552 ～ 11.04 mg·L -1 of flagy and 0.4 ～ 8.0 mg·L -1 of tinidazol ( r = 0.9999 , r = 0.9999 , r = 0.9998 ).The average recovery was respectively 99.41% , 98.64% and 100.26% . The relative standard deviation of intra day and inter day was less than 4.0% . CONCLUSIONS The established method can be used for determination of the drugs mentioned in bile.

关 键 词：氟罗沙星 甲硝唑 替硝唑 高效液相色谱法 胆汁 

分 类 号：R927.2[医药卫生—药学]