细胞外基质促血管平滑肌细胞迁移及β_3整合素和粘着斑激酶表达  

The relationship between expression of β_3 integrin, focal adhesion kinase and migration of vascular smooth muscle cells stimulated by extracellular matrix

作　　者：刘智敏[1] 韩梅[1] 温进坤[1] 

机构地区：[1]河北医科大学基础医学研究所河北省医学生物技术重点实验室,河北石家庄050017

出　　处：《中国病理生理杂志》2003年第2期163-166,共4页Chinese Journal of Pathophysiology

基　　金：国家自然科学基金资助项目 (No.39970 2 74) ;河北省自然科学基金资助项目 (No .30 1 358)

摘　　要：目的 :研究细胞外基质 (ECM)蛋白促血管平滑肌细胞 (VSMC)迁移与 β3整合素及粘着斑激酶 (FAK)表达之间的关系。方法 :应用细胞迁移实验观察骨桥蛋白 (OPN)和纤连蛋白 (FN)对VSMC迁移活性的影响 ,利用RT-PCR和Western印迹探讨 β3整合素、FAK和转录因子Gax表达与细胞迁移之间的关系。结果 :VSMC经 1 0、2 0mg/LOPN和 2 0mg/LFN处理后 ,迁移活性分别达对照组的 1 36、1 57和 1 2 6倍 (P <0 0 5)。用相同浓度 (2 0mg/L)OPN和FN刺激细胞 ,随着作用时间延长 ,VSMC迁移距离逐渐增加。Western印迹和RT -PCR结果证实 ,两种基质蛋白促进VSMC迁移与诱导 β3整合素和FAK基因表达及抑制转录因子Gax表达有关。结论 :β3整合素AIM: To study the relationship between expression of β 3 integrin, focal adhesion kinase (FAK) and migration of vascular smooth muscle cells (VSMC) stimulated by osteopontin (OPN) and fibronectin (FN).METHODS: VSMC migration was examined using a model of wounding injury of confluent cultured cells. The expression of β 3 integrin, FAK and Gax genes in VSMC were detected using Western blotting and RT-PCR. RESULTS: OPN and FN induced the migration activity of VSMC in a time-dependent manner ( P<0 05 ). Western blotting and RT-PCR showed that both OPN and FN up-regulated the expression of β 3 integrin and FAK, but reduced the expression of Gax during migration of VSMC under the same conditions. CONCLUSION: β 3 integrin-FAK is one of the signaling pathways mediated VSMC migration stimulated by OPN and FN.

关 键 词：细胞外基质蛋白 血管平滑肌 结合素类 局部粘着 Β3整合素 粘着斑激酶 

分 类 号：Q249[生物学—细胞生物学]