TF-1细胞凋亡相关基因19在系统性红斑狼疮发病中的作用  

The role of TF-1 cell apoptosis-related gene 19 in systemic lupus erythematosus

作　　者：宋清华[1] 王晶[1] 陈英玉[2] 狄春晖[2] 孙荣华[2] 陈学荣[1] 李世荫[1] 

机构地区：[1]北京大学第三医院皮肤科,北京100083 [2]北京大学医学部免疫系,北京100083

出　　处：《中国病理生理杂志》2003年第2期189-193,T004,共6页Chinese Journal of Pathophysiology

基　　金：中华医学会皮科学科研基金 (诺华基金)资助项目

摘　　要：目的 :探讨TF - 1细胞凋亡相关基因 1 9(TFAR1 9)在系统性红斑狼疮 (SLE)发病诱因中的作用及其与SLE病情的关系。方法 :应用倒置相差显微镜、Ladder方法、Westernblotting方法、荧光免疫显微镜、ELISA方法检测中波紫外线 (UVB)诱导HaCaT细胞凋亡时TFAR1 9的表达及其与SLE患者病情的关系。结果 :在剂量为 30mJ/cm2的UVB照射 2 4h后 ,HaCaT细胞开始凋亡 ,TFAR1 9在此过程中有表达 ,发现活动期SLE患者血清中TFAR1 9的抗体值高于稳定期和正常对照组的值 (P≤ 0 .0 5)。结论 :在UVB诱导角质形成细胞HaCaT凋亡的过程中 ,有TFAR1 9的参与并发挥了作用 ,提示TFAR1AIM: To clarify the role of new apoptosis-related gene, TF-1 cell apoptosis-related gene 19(TFAR19), in the pathogenesis of systemic lupus erythematosis (SLE) and the relationship between TFAR19 and SLE. METHODS: DNA Ladder detection, Western blotting, immunological fluorescence method, ELISA and so on were used to test if ultraviolet B(UVB) could induce HaCaT cell apoptosis and TFAR19 expression. RESULTS: HaCaT cell apoptosis could be detected after 24 hours of 30 mj/cm 2 UVB irradiation. Also, we found that in active SLE patients, the TFAR19 antibody was increased, but not significant compared to the normal control. CONCLUSION: TFAR 19 is involved in the process of UVB induced ketatinocyte line HaCaT apoptosis and SLE pathogenesis.

关 键 词：系统性红斑狼疮 细胞凋亡 紫外线B TF-1细胞凋亡相关基因19 

分 类 号：R593.241[医药卫生—内科学]