人参二醇组皂甙对游泳训练大鼠红细胞膜流动性的影响  

Effect of panaxadiol saponins on fluidity of red blood cell membrane in heavy load swimming trained rats

作　　者：杨柯[1] 李峰[1] 王志农[1] 赵雪俭[1] 徐桂香[2] 陈燕萍[3] 马兴元[3] 

机构地区：[1]吉林大学基础医学院生殖病生研究室,吉林长春130021 [2]吉林省计划生育科研所不孕不育治疗中心 [3]吉林大学基础医学院天然药物化学研究室,吉林长春130021

出　　处：《中国病理生理杂志》2003年第2期249-252,共4页Chinese Journal of Pathophysiology

基　　金：卫生部科研基金资助项目 (No .98- 2 - 1 82 )

摘　　要：目的 :探讨人参二醇组皂甙 (PDS)防治长期耐力运动引起的红细胞损伤的作用。方法 :稳态荧光偏振技术测定 7周大运动量递增负荷游泳训练大鼠红细胞膜流动性 ,邻苯三酚 -氯化硝基四氮唑蓝法测定红细胞超氧化物歧化酶活性 ,硫代巴比妥酸比色法测定血清丙二醛 (MDA) ,扫描电镜观察红细胞形态。结果 :运动·生理盐水组大鼠红细胞膜脂荧光偏振度 (polatization ,P值 )显著高于安静对照组 ,反映红细胞膜流动性低于安静对照组 (P<0 0 5) ;运动·生理盐水组的血清MDA水平显著高于安静对照组 (P <0 0 5) ,而RBC -SOD活性显著低于安静对照组 ;运动·PDS组P值显著低于运动·生理盐水组 ,红细胞膜脂流动性高于运动·生理盐水组 (P <0 0 5) ;运动·PDS组血清MDA水平显著低于运动·生理盐水组 (P <0 0 1 ) ,RBC -SOD活性显著高于运动·生理盐水组 (P <0 0 5) ;运动·生理盐水组的RBC计数、Hb、MCHC、Hb指数显著低于安静对照组 (P <0 0 5) ;而运动·PDS组RBC计数、Hb、MCHC、Hb指数显著高于运动·生理盐水组 (P <0 0 5) ;扫描电镜观察到运动·生理盐水组红细胞呈口形 ,棘形 ,嵴形 ,靶形等异常改变 ,而运动·PDS组红细胞形态基本正常。结论 :PDS可降低运动训练后血清和红细胞脂质过氧化产物的形成 。AIM: To study effects of prolonged intensive endurance exercise and Panaxadiol saponins (PDS) on red blood cells (RBC) membrane fluidity in rats, as well as the mechanism. METHODS: By the membrane fluorence polarization, Pyrollol-NBT, TBA methods and Scanning Electron Microscope, RBC membrane fluidity,RBC-SOD and serum MDA were measured after 7 weeks prolonged intensive swimming. RESULTS: Fluorence polarization(P value) were significant higher and fluidity were lower( P <0 05), at the same time RBC-SOD activities lower and MDA contents higher in exercise-saline group than that of normal control group( P <0 05). P value were lower and fluidity were higher ( P <0 05), at the same time, RBC-SOD activities lower and MDA contents higher in exercise-PDS group than that of exercise- saline group( P <0 05). RBC count, Hb, MCHC and Hb index in exercise- saline group were significant lower than that of normal control group( P <0 05) and those parameter in exercise- PDS group were significant lower than that of exercise- saline group( P <0 05).Morphological study by scanning electron microscope showed that abnormal erythrocytes were found in exercise-saline group, while seldomly found in exercise-PDS group and normal control groups. CONCLUSION: PDS might reduce the lipid peroxidation in serum and RBC and has a good protective effect against RBC injury induced by prolonged intensive endurance exercise.

关 键 词：运动性贫血 人参 红细胞 膜流动性 大鼠 

分 类 号：R556[医药卫生—血液循环系统疾病]