内毒素血症时大鼠肝细胞线粒体损伤及其机制的研究  

Injury of hepatic mitochondria and its pathogenesis in rats with endotoxemia

作　　者：毕铭华[1] 张淑文[1] 王宝恩[1] 刘树森[2] 宋伟[2] 

机构地区：[1]北京友谊医院感染内科,北京100050 [2]中国科学院动物研究所生物膜与膜生物国家重点实验室,北京100050

出　　处：《中国病理生理杂志》2003年第2期266-268,共3页Chinese Journal of Pathophysiology

摘　　要：目的 :观察急性感染性内毒素血症大鼠肝细胞线粒体呼吸链的损伤及其机制。方法 :将体重在 2 50- 2 80g健康SD大鼠随机分为空白对照组、内毒素组。提取内毒素血症大鼠肝细胞线粒体 ,测定其超氧阴离子O 2生成量 ,同时测定线粒体呼吸链功能 :复合体Ⅱ +Ⅲ的电子传递与质子转移定量关系 (H+ / 2e- )、ADP/O、呼吸控制率(RCR)。结果 :内毒素血症大鼠肝细胞线粒体电子漏显著增加 ;大鼠肝细胞线粒体以琥珀酸为底物的态 4和态 3呼吸速率增加 ,ADP/O、RCR及复合体Ⅱ +Ⅲ的H+ / 2e- 显著降低。结论AIM: To investigate the effect of endotoxin on rat hepatic mitochondria. METHODS: Rats were randomly divided into two groups:endotoxin group and the control. 8 cases of animals were included in each group. The effect of electron leak on the production of endogenous oxygen free radicals and the changes of mitochondria function were studied.RESULTS: Treated with endotoxin, a significant increase in O  2 and the rate of state 3,4 were observed in liver mitochondria; The rate of electron transfer to proton pump of mitochondria respiratory chain complex Ⅱ+Ⅲ( H +/2e -), respiratory control rate and ADP/O decreased significantly. CONCLUSION: A increase in production of endogenous oxygen free radicals induced by endotoxin plays an important role in the injury of rat liver mitochondria.

关 键 词：线粒体 肝 内毒素类 大鼠 

分 类 号：R333.4[医药卫生—人体生理学]