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作 者:叶创兴[1] HIROSHI Ashihara 郑新强 王秀娟[1] 高昆[1] 张宏达[1]
机构地区:[1]中山大学生命科学学院,广东广州510275 [2]Department of Biology
出 处:《中山大学学报(自然科学版)》2003年第1期62-65,共4页Acta Scientiarum Naturalium Universitatis Sunyatseni
基 金:国家自然科学基金资助项目 ( 395 70 0 81)
摘 要:通过单株定性和定量的分析 ,发现中国特有的变种苦茶Camelliaassamicavar kuchaChangetWang其芽叶嘌呤碱依含量高低分别由 1 3 7 9_四甲基尿酸、咖啡碱、可可碱组成 ,其中 1 3 7 9_四甲基尿酸的含量占芽叶干质量的 1 3%~ 3 4% ,其次是咖啡碱 ,其质量分数为 0 2 6 %~ 2 8% ,再其次是可可碱 ,质量分数为 0 1%~2 1%。苦茶嘌呤碱各组成成份在植株间存在差别 ,但多数植株 1 3 7 9_四甲基尿酸始终表现为优势嘌呤碱 ;少数植株咖啡碱表现为优势嘌呤碱 ,而 1 3 7 9_四甲基尿酸是第二位的嘌呤碱。在调查植株间嘌呤碱时还发现有的植株时而表现为 1 3 7 9_四甲基尿酸是优势嘌呤碱 ,时而表现为咖啡碱是优势嘌呤碱。这些现象产生的真正原因仍然有待于研究 ,但苦茶嘌呤碱的组成模式是有花植物中尚未发现的。The composition of purine alkaloids in kucha (Camellia assamica var. kucha Chang et Wang) were investigated from its one buds and two leaves including cultivated and wild samples in detail by qualitative TLC and quantitative HPLC, the results indicate that major purine alkaloids were 1,3,7,9-tetramethyluric acid (theacrine) and caffeine. Theacrine in most of individuals inner variety was the major purine alkaloid and its content was from 1.3% to 3.4% dried weight. And theacrine in kucha was always companied with caffeine and theobromine. The concentration of theacrine was highest in apex buds, and then were rapidly decreased from young, mature to aged leaves. The theacrine content were changed together with seasons but its characterization of theacrine as major purine alkaloid were not changed in identified individual trees with major theacrine. In some individual trees, the theacrine content was changeable, sometime major purine was theacrine and otherwise was caffeine. Containing predominant theacrine in kucha is a new record not only in taxa of genus Camellia Section Thea(L.)Dyer but also is a new record in flowering plants.
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