复方红景天干预大鼠肝纤维化作用的实验研究  

The protective effects of compound Rhodiola sachalinensis A Bor on CCL_4 induced liver fibrosis in rats

作　　者：曾维政[1] 吴晓玲[2] 蒋明德[1] 陈晓斌[1] 

机构地区：[1]成都军区总医院消化内科,成都610083 [2]重庆医科大学

出　　处：《胃肠病学和肝病学杂志》2003年第1期24-29,共6页Chinese Journal of Gastroenterology and Hepatology

基　　金：成都军区"十五"医药卫生科研基金资助项目(0 1A0 0 9)

摘　　要：目的　从血清、组织、分子等水平探讨复方红景天抗大鼠肝纤维化的作用及其可能的机制 ,为抗肝纤维化研究提供新的选择。方法　用四氯化碳皮下注射法诱导SD大鼠肝纤维化模型 ,同时给予复方红景天颗粒口服进行干预性治疗 ,观察大鼠肝功能 ,血清纤维化相关酶活性ALT、AST、β NAG、MAO ,血清肝纤维化指标PCⅢ、CIV、HA以及肝组织羟脯氨酸含量和病理学变化 ,同时检测复方红景天对大鼠肝组织TGFβ1、Ⅰ型前胶原mRNA表达水平的影响。 结果　口服复方红景天可改善大鼠肝功能 ,降低血清PCⅢ、CⅣ、HA水平 ,抑制 β NAG、MAO的活性 ,降低肝组织羟脯氨酸含量 ,明显改善大鼠肝组织病理变化 ,并降低大鼠肝组织TGFβ1、Ⅰ型前胶原mRNA的表达水平。 结论　复方红景天可能通过抑制TGFβ1、Ⅰ型前胶原mRNA的表达从而减少胶原合成、保护肝细胞等机制有效干预CCl4 诱导的大鼠肝纤维化 。Aim To study the protective effects of compound Rhodiola sachalinensis A Bor on CCl 4 induced liver fibrosis in rats.Methods Male SD rats were divided into 3 groups:nomal(N),compound Rhodiola sachalinensis A Bor(R) and model(M) group.The M group and R group were given 30% CCl 4 (3 mL/kg,2 times/week) for 14 weeks and compound Rhodiola sachalinensis A Bor (0.5 g/kg,p.o.,daily for 14 weeks )was administered to rats of R group.The changes of serum ALT,AST,β NAG,MAO and PCⅢ,CⅣ,HA were assayed.The liver histopathological changes were observed with HE、Masson staining.The concentration of hydroxyproline in the liver tissue was examined with Jammal method.The expression of TGF β1 mRNA and (α1) I collegen mRNA were observed with semi quantified in situ RT PCR.Results Compound Rhodiola sachalinensis A Bor treatments decreased the serum level of ALT,AST,β NAG,MAO,PCⅢ,CⅣ,HA.It reduced the concentration of hydroxyproline in the liver tissue.The reduction of the enzymes activities in serum correlated with the improvement of liver histology.At the same time,it inhibited the expression of TGF β1 mRNA and (α1) I collagen mRNA.Conclusions Compound Rhodiola sachalinensis A Bor is effective in protecting against CCl 4 induced liver fibrosis in rats.The machanisms of the protection appear to due to the regulation of collagen production and the protection of hepatic cells.

关 键 词：复方红景天 大鼠 肝纤维化 实验研究 抑制 

分 类 号：R259.752[医药卫生—中西医结合]