粘附分子基因表达在大鼠急性坏死性胰腺炎肺损伤中的作用  

Intrapulmonary expression of intercellular adhesion molecule-1 gene in rats with acute necrotizing pancreatitis

作　　者：朱斌[1] 孙家邦[1] 张淑文[1] 李非[1] 刘爽[1] 崔叶青[1] 孙海晨[1] 

机构地区：[1]首都医科大学北京宣武医院普外科,100053

出　　处：《中华普通外科杂志》2003年第1期20-22,共3页Chinese Journal of General Surgery

基　　金：北京市卫生局重点学科资助项目

摘　　要：目的　研究急性坏死性胰腺炎 (ANP)大鼠肺组织细胞间粘附分子 1(ICAM 1)mRNA的表达情况及其与肺损伤的关系。方法　将 33只Wistar大鼠随机分为正常对照组和胰腺炎不同时间点 (1、4、12和 2 4h)各组 ,用逆行胰胆管注射方法制备ANP模型。采用逆转录聚合酶链反应法检测ANP大鼠肺组织中ICAM 1mRNA表达 ,同时观察肺组织髓过氧化物酶 (MPO)及病理改变。结果造模ANP 1h后大鼠肺组织中ICAM 1mRNA(0 82± 0 0 3)比正常对照组 (0 41± 0 0 4)的表达水平高 (P <0 0 5 ) ,并持续升高至 12h及 2 4h(分别为 1 17± 0 0 5及 1 11± 0 0 4) ,同时伴有肺组织病理损害 ,其严重程度与ICAM 1mRNA表达、MPO及肺MPO与ICAM 1mRNA表达均呈正相关 ,相关系数分别为0 85、0 85及 0 96 (P <0 0 5 )。结论　ANP大鼠早期肺组织中ICAM 1mRNA呈过度表达 ,肺损伤严重程度与ICAM 1mRNA表达的高低有关。肺组织中ICAM 1过度表达及中性粒细胞浸润是ANP肺损害发生的原因之一。ObjectiveTo investigate intrapulmonary expression of ICAM-1 mRNA and the relationship between the expression of ICAM-1 and lung injury in ANP. Methods ANP was reproduced by retrograde injection of 3.5% sodium taurocholate. Animals were sacrificed at 1,4,12 and 24 hours after induction of pancreatitis. Intrapulmonary ICAM-1 mRNA expression was assayed by reverse transcription-PCR. The following parameters were also measured: Serum amylase, histologic grading of lung injury and intrapulmonary myeloperoxidase (MPO). ResultsThere was intrapulmonary overexpression of the ICAM-1 mRNA on the first hour and persisted up to 12-24 hours with the increase of serum amylase and intrapulmonary MPO after pancreatitis induction. Score of the lung injury correlated well with the expression of the ICAM-1 mRNA and intrapulmonary MPO (r=0.85, 0.85 and 0.96, all P<0.05).ConclusionThe expression of ICAM-1 mRNA and pulmonary leucocyte infiltration was related with lung injury in ANP. Severity of pancreatitis and lung injury could be predicted by the expression of ICAM-1 mRNA in ANP.

关 键 词：粘附分子 基因表达 大鼠 急性坏死性胰腺炎 肺损伤 

分 类 号：R657.51[医药卫生—外科学]