质子对小麦的诱变效应及作用机理研究 Ⅱ质子处理对小麦种胚DNA损伤修复的影响  

STUDIES ON MUTAGENIC EFFECTS AND MECHANISM OF WINTER WHEAT IRRADIATED BY PROTON Ⅱ.Unscheduled DNA Synthesis of Irradiated Wheat seeds

作　　者：施巾帼[1] 孙国庆[1] 胡萍[1] 肖京城[1] 李桂英[1] 唐掌雄[1] 

机构地区：[1]中国农业科学院原子能利用研究所,北京100094

出　　处：《核农学报》2003年第1期1-5,共5页Journal of Nuclear Agricultural Sciences

基　　金：国家自然科学基金项目 (39870 4 4 5 )资助

摘　　要：用3H TdR标记小麦种胚 ,研究了质子处理的种胚萌发早期DNA合成动态 ,并与γ射线辐照进行对比。表明质子处理能引起肿胚DNA损伤修复合成 ,即DNA非按期合成 ,京 41 1与原冬 977的修复复制峰期分别出现在正常的DNA合成峰期前 4h与 2h。以京 41 1为例 ,比较质子处理与γ射线辐照的种胚DNA损伤修复特点的差异 ,质子的DNA非按期合成峰期出现时间早于γ射线处理的 2h ,正常的DNA合成峰期晚于γ射线处理的 4h ,且在DNA非按期合成峰期后的DNA合成量明显低于γ射线。By 3H\|TdR incorporation the changes of DNA synthesis in the early period of wheat seeds germination irradiated with proton were studied.The results showed that proton was able to induce an unscheduled DNA synthesis(UDS) in seed embryos of wheat.The UDS occurred earlier than the normal DNA synthesis,2h and 4h in Yuandong 977 and Jing411 respectively.Compared with γ\|ray treatment,the UDS in seed embryos by proton appeared 2h earlier and the normal DNA synthesis 4h later.Also the level of DNA synthesis was significantly lower than that treated by γ\|ray irradiation after UDS.

关 键 词：质子 小麦 诱变效应 作用机理 ^3H-TdR标记 损伤修复 DNA合成 

分 类 号：S512.1[农业科学—作物学]