连续及部分睡眠剥夺96小时后大鼠脑干中c-Fos蛋白的表达  

c-Fos Protein Expression of the Rats on the Cerebral Stem after Continuing and Partial Sleep Deprivation of 96 Hours

作　　者：宋国萍[1] 苗丹民[1] 皇甫恩[1] 陈足怀[1] 

机构地区：[1]第四军医大学航空航天医学系心理教研室,陕西西安710032

出　　处：《中国临床心理学杂志》2003年第1期9-11,共3页Chinese Journal of Clinical Psychology

摘　　要：目的 :探索连续及部分不同睡眠剥夺条件下的脑c -Fos蛋白的表达。方法 :采用小平台水环境法建立大鼠睡眠剥夺模型 ,用Fos蛋白免疫组化的方法分别测量连续睡眠剥夺 96小时组、部分睡眠剥夺 96小时组、大平台对照组和正常单独饲养组脑干中Fos蛋白的表达 ,每组 4只。结果 :同连续睡眠剥夺组相比 ,部分睡眠剥夺组表达范围更广 ,在脑干网状结构及中缝的被盖背侧核、脑桥被盖网状核及脑桥吻侧网状核、脑桥尾侧网状核、丘脑的中线核群、板内核群和网状核均有所表达 ,但阳性程度低。结论 :部分睡眠剥夺对脑的影响要小于连续睡眠剥夺。Objective:To investigate the expression of c-Fos protein after total and partial sleep deprivation(SD) of 96 hours.Methods:SD was induced in the male Sprague-Dawley rats by housing them on the small platforms over water.Controls were housed in the normal cage(CC) or on the large platforms over water(TC).The effects of total and partial 96h SD on the expression of c-Fos protein were examined by immunocytochemisty.Results:SD made Fos protein express differently in the different fields of the brain.The expression of Fos protein of partial SD was wider and lighter than of total SD.Conclusion:Compared with total SD,partial SD has lighter stress.

关 键 词：C-FOS蛋白 脑干 睡眠剥夺 免疫组织化学 动物实验 

分 类 号：R338.63[医药卫生—人体生理学] R392[医药卫生—基础医学]