制备高效价细菌免疫血清的实验研究  

Laboratory Study on Preparation of High Valent Germ Immunoserum

作　　者：罗映辉[1] 文质[1] 陈利玉[1] 刘水平[1] 夏忠弟[1] 

机构地区：[1]中南大学湘雅医学院微生物教研室,中国湖南长沙410078

出　　处：《实用预防医学》2003年第1期23-24,共2页Practical Preventive Medicine

基　　金：湖南省高等学校重点学科经费资助

摘　　要：目的　制备高滴度的细菌免疫血清 ,开展肥达氏反应的教学实验 ,节约经费 ,保证教学实验质量的提高。　方法　行颅内、脚掌、皮下注射采用弗氏不完全佐剂 +死或活细菌 ,及耳静脉注射途径注射细菌于家兔体内 ,制备免疫血清 ,测四种不同注射途径和两种注射法的抗体效价。　结果　四种注射途径的效价无差异。　结论　耳静脉注射法安全方便 ,操作易控制 。Objective To prepare multivalent germ immunoserum, and develop the teaching of Widal test so as to save funds and grarantee the teaching experiments. Methods To give incomplete Freunds adjurant+dead or living bacteria by inskin, foot, subcutaneous or ear-vein injection. Result The valences of the four injection ways have no difference. Conclusion Ear-vein injection method is safe, convenient, and easy to control, which is better than others.

关 键 词：高效价细菌免疫血清 注射法 耳静脉法 抗体效价 

分 类 号：R392－33[医药卫生—免疫学]