痕量有机汞形态的反相高效液相色谱研究  

Studies on Trace Organomercury Speciation by RP-HPLC

作　　者：江霜英[1] 刘锦春[1] 刘良斌[1] 程介克[1] 

机构地区：[1]武汉大学化学系,武汉430072

出　　处：《高等学校化学学报》1992年第6期759-761,共3页Chemical Journal of Chinese Universities

基　　金：国家自然科学基金

摘　　要：汞是重要的污染元素之一,尤以有机汞的毒性更大.早期的研究工作,大多采用气相色谱法,它要求试样必须具有挥发性和热稳定性,因而在应用上受到一定限制.近年来有关高效液相色谱分析的报道逐渐增多,柱前衍生和柱中衍生化技术均有应用,常用的衍生化试剂有APDC、DDTC和双硫腙等. 铋试剂Ⅱ是一种性能优良的广谱性螯合剂,对汞有良好的络合性能.我们将它用作有机汞的柱前衍生化试剂,在C_8柱上以正丁醇-甲醇-水作流动相,苹果酸为辅助络合剂,分离和定量测定了有机汞的3种形态,检测限分别为1.4ng(MeHg^+)、1.0ng(EtHg^+)和2.5ng(PhHg^+),并成功地应用于东湖水样的分析.With precolumn derivatization by Bismuthiol Ⅱ , MeHg+, EtHg+ and PhHg+ were quantitatively separated and determined by RP-HPLC on a Shimadzu CLC-C8 column. A bu-tanol-methanol-water mixture was used as the mobile phase, and malic acid was chosen as an auxiliary complexant. The detection limits are 1. 4 ng(MeHg+), 1. 0 ng(EtHg+) and 2. 5 ng (PhHg+), respectively. The method was applied to speciation analysis of organomercury in environmental water.

关 键 词：铋试剂Ⅱ 有机汞 形态分析 RP-HPLC 

分 类 号：O627.23[理学—有机化学]