HPLC法测定泻痢停片中醋酸泼尼松的含量  

Determination of prednisone acetate in Xie Li Ting tablets by HPLC

作　　者：钟颖[1] 曾志坚[1] 

机构地区：[1]江门市药品检验所,广东529000

出　　处：《广东药学》2003年第1期17-18,共2页Guangdong Pharmaceutical Journal

摘　　要：目的　建立泻痢停片中醋酸泼尼松的高效液相检测法。方法　色谱柱为HypersilODS2 (5 μm ,4.6mm× 2 0 0mm) ,流动相为甲醇 -水 (5 8∶42 ) ,流速 1.0ml min ,检测波长 2 40nm ,氢化可的松为内标物。结果　醋酸泼尼松和内标的保留时间分别为 11.4min和 7.5min。在 80～ 2 40 μg ml浓度范围内 ,醋酸泼尼松与内标峰面积之比和浓度呈良好的线性关系 (r =0 .9998) ,醋酸泼尼松平均回收率为 99.92 % ,RSD为 0 .2 4%。结论　用高效液相色谱法测定醋酸泼尼松的含量 ,有简便、准确、灵敏度高的优点 。Objective To develop a method for analysis of prednisone acetate in Xie Li Ting tablets by HPLC. Methods Separation was obtained by using a hypersil ODS2 (5μm,4.6mm×200mm).The mobile phase consisted of a mixture of methanol water(58 ∶ 42) and the flow rate was 1.0ml/min. The determination warelength was 240nm,using hydrocortisong as internal standard. Results The retention times of prednisone acetate and IS were 11.4 and 7.5min respectively. A linear relationship was obtained between the peak area ratio and the concentration of prednisone acetate from 80 to 240μg·ml -1 . The average recoveries of prednisone acetate was 99.92%,with RSD of 0.24%. Conclusion The method is simple,accurate and sensitve and can be used in determination of prednisone acetate in Xie Li Ting tablets.

关 键 词：HPLC法 泻痢停片 醋酸泼尼松 含量测定 

分 类 号：R975.3[医药卫生—药品] R927.2[医药卫生—药学]