高效液相色谱法测定草乌中乌头碱含量  

Determination of brucine and strychnine in Aconitum Kusnezoffii Reichbby RP-HPLC

作　　者：王朝虹[1] 何毅[1] 张继宗[1] 胡春华[1] 

机构地区：[1]公安部物证鉴定中心,北京100038

出　　处：《刑事技术》2003年第1期15-16,共2页Forensic Science and Technology

基　　金：公安部资助课题(No.20018223301)

摘　　要：目的建立反相高效液相色谱方法定量分析药材生草乌中乌头碱的含量。方法XTerraTMRP185μm,(150mm×4.6mm)色谱柱;流动相为乙腈:10mmol·L-1碳酸氢铵50∶50(V/V)等度洗脱;流速1.0ml/min;检测波长230nm。结果乌头碱的线性范围为5～20μg,最小检出浓度为0.5μg/ml。结论该方法灵敏、快速、准确,适用于生药、饮片、药酒中乌头碱的检测。Objective To establish a RP-HPLC analysis method for determination of aconitine in Aconitum Kusnezoffii Reichb.Methods XTerra TM RP 18 column 5μm,(150mm×4.6mm);the mobile phase consisting of acetonitrile:10mmol ammonium hydrogen carbonate buffer50:50(V/V),a flow rate of1.0ml ·min -1 and detection wave length at230nm.Results:The linear range of aconitine was5～20μg and the limit of detection was0.5μg /ml.Conclusion This method was sensitive,fast and accurate.

关 键 词：草乌 乌头碱 高效液相色谱 毒物检验 含量测定 

分 类 号：D919.1[医药卫生—法医学]