小鼠吸入香烟烟雾染毒过程的标准化和使用酶活检测在烟熏小鼠中基因CYP1A1的表达(英文)  

Standardization of Administration Procedure for Mice Exposed to Cigarette Smoke and the CYP1A1 Gene Expression Detected by an Enzyme Activity

作　　者：周仁清[1] 沈英娃 周樱桥[3] 薄涛[1] 沙莉[1] 阿尔夫瑞德.豪葛 

机构地区：[1]辽宁大学生命科学系,辽宁沈阳110036 [2]国家环境保护化学品登记中心,北京100012 [3]中国科学院沈阳生态研究所,辽宁沈阳110015 [4]美国密执安州立大学微生物系,西兰辛48824

出　　处：《辽宁大学学报（自然科学版）》2003年第1期1-6,共6页Journal of Liaoning University：Natural Sciences Edition

摘　　要：建立了一个通过测定茄尼醇的含量来确定小鼠吸入香烟烟雾浓度的方法 ,因而使得来自不同实验室的相关数据的比成为可能 .同时 ,对香烟烟雾诱导雄性小鼠NMR1的肺微粒体中细胞色素P4 5 0异构基因CYP1A1的表达在蛋白质的水平上进行了测试 .测试结果表明 ,代表CYP1A1蛋白活性的 7 乙氧基试卤灵 O 去乙基酶的活性在小鼠的肺中随着吸入香烟烟雾的增加而上升 ;由此可知 ,香烟烟雾诱导了小鼠肺中基因CYP1A1的表达 .We set up an experiment in which the concentration of cigarette smoke exposed to mice was able to be determined by detecting the concentration of solanesol. This standardized cigarette smoke expsoing ,method can make the data from different laboratories be comparable. At the same time, the pulmonary microsomes of male NMR1 mice were used to study the inductive effect of tobacco smoke on cytochrome P450 isoform CYP1A1 gene expression at the protein level. The CYP1A1 protein activity was evaluated by the catalytic activity of 7 ethoxyresorufin O deethylase (EROD). The results showed that the pulmonary microsomal EROD activity was elevated in the mice exposed to cigarette smoke.

关 键 词：香烟烟雾 染毒过程 标准化 酶活检测 茄尼醇 CYP1A1基因 小鼠 肺 基因表达 烟雾浓度 

分 类 号：R163[医药卫生—公共卫生与预防医学] Q786[生物学—分子生物学]