大鼠心肌成纤维细胞合成金属硫蛋白  

The synthesis of MT in rat myocardial fibroblasts

作　　者：高霖[1] 王冬艳[1] 李扬[2] 石彦荣[1] 卜定方[1] 庞永正[1] 唐朝枢[1] 

机构地区：[1]北京大学第一医院心血管病研究所,北京100034 [2]北京大学医学部临床医学系

出　　处：《北京大学学报（医学版）》2003年第1期41-44,共4页Journal of Peking University:Health Sciences

基　　金：国家重点基础研究发展规划项目 (G2 0 0 0 5 690 5 );北京大学 985项目资助~~

摘　　要：目的 :探讨脂多糖 (lipopolysaccharide ,LPS)、碱性成纤维细胞生长因子 (basicfibroblastgrowthfactor ,bFGF)、氧化型低密度脂蛋白 (oxidizedlow densitylipoproteins ,oxLDL)及重金属物质ZnCl2 等刺激因素对大鼠心肌成纤维细胞金属硫蛋白 (metallothionein ,MT)合成的影响。方法 :用10 9Cd Hb饱和法测定细胞MT含量 ,用RT PCR法测定细胞MTmRNA含量。结果 :本实验中各种处理因素均可刺激心肌成纤维细胞MT含量的增加。 1和5mg·L-1LPS处理组成纤维细胞MT含量与对照组相比分别增加 94 %和 130 % (P <0 .0 1)。 0 .2和 1.0mg·L-1bFGF处理组分别增加 4 1%和 5 9% (P <0 .0 1)。oxLDL亦可以促进心肌成纤维细胞MT的合成 ,2和 10mg·L-1oxLDL组MT含量较对照组分别增加 2 3% (P <0 .0 5 )和 4 1% (P <0 .0 1)。 1,2 ,5mg·L-1ZnCl2 呈浓度依赖性刺激心肌成纤维细胞MT含量的增加 ,与对照组相比分别增加 6 6 %、99%和 14 9% (P <0 .0 1) ,2～ 8mg·L-1ZnCl2 诱导MT1mRNA含量增加 6 9%～ 12 9% (P <0 .0 1) ,MT2mRNA含量增加 31%～ 6 0 % (P <0 .0 1)。但 10mg·L-1ZnCl2 不能进一步增加细胞MT含量 ,仅较对照组增加 91% ,可能与高浓度ZnCl2 致细胞损伤 ,细胞存活率降低有关。结论 :LPS、bFGF、oxLDL、ZnCl2 等多?Objective: To study the influence on the synthesis of metallothionein (MT) induced by lipopolysaccharide (LPS), basic fibroblast growth factor (bFGF), oxidized low density lipoproteins (oxLDL) and ZnCl 2 in rat myocardial fibroblasts.Methods: The content of MT was measured by 109 Cd Hb saturation method and RT PCR was used to determine the expression of MT mRNA. Results: All of the stimulus in this experiment increased the synthesis of MT in myocardial fibroblasts. Compared with control, 1 and 5 mg·L -1 LPS increased the content of MT by 94% and 130% ( P <0.01), 0.2 and 1.0 mg·L -1 bFGF by 41% and 59% ( P <0.01), 2 and 10 mg·L -1 oxLDL by 23% ( P < 0.05) and 41% ( P <0.01) respectively. 1,2 and 5 mg·L -1 ZnCl 2 increased the content of MT by 66%,99% and 149% ( P <0.01) in a concentration dependent manner. 2-8 mg·L -1 ZnCl 2 increased the content of MT1 mRNA by 69%-129%, MT2 mRNA by 31%-60% ( P <0.01). But 10 mg·L -1 ZnCl 2 didn't stimulate the synthesis of MT more significantly, probably because ZnCl 2 of high concentration damaged myocardial fibroblasts and decreased the survival rate of the cells. Conclu sion: Many stimulus such as LPS, bFGF, oxLDL and ZnCl 2 can induce the synthesis of MT ,and ZnCl 2 can increase the expression of MT mRNA in rat myocardial fibroblasts.

关 键 词：心肌 成纤维细胞 金属硫蛋白 生物合成 

分 类 号：Q26[生物学—细胞生物学]