季节因素对大鼠睾丸黄体生成素受体影响的实验研究  

The seasonal effect on luteinizing hormone receptor of rat's testes

作　　者：卢全生[1] 郭霞珍[2] 贺师鹏[3] 李悦山[4] 

机构地区：[1]中国中医研究院西苑医院,北京100091 [2]北京中医药大学,北京100029 [3]北京大学医学部,北京100083 [4]新疆石河子大学医学院,石河子832002

出　　处：《生殖医学杂志》2003年第1期24-29,共6页Journal of Reproductive Medicine

基　　金：国家自然科学基金资助课题 ( 3 9770 888)

摘　　要：目的　探索季节变化对大鼠睾丸细胞膜黄体生成素 ( L H)受体的影响及其生理机制。　方法　采用大鼠松果体摘除模型 ,运用放射配体结合分析 ( RBA)方法测定冬夏季节大鼠睾丸细胞膜 LH受体变化。　结果　正常组睾丸细胞膜 L H受体最大结合量和结合力在冬夏季节存在明显差异 ( P<0 .0 1 ) ,表现出冬高夏低的变化 ;松果体摘除模型组睾丸细胞膜 L H受体最大结合量和结合力在冬夏季节无明显差异 ;正常组和模型组之间睾丸细胞膜 L H受体数目存在显著差异。　结论　季节因素对 L H受体蛋白的表达有调节作用 ;松果体可能是 LObjective: To explore the seasonal effect on luteinizing hormone (LH) receptor of rat testicular cells and its mechanism. Method: The animals were divided into two groups: normal group and pinealectomy group. The specific binding of luteinizing hormone to rat testicular cells was measured by the radioligand binding assay (RAB). Results: In the normal rats, the maximal binding capacity (Bmax) and equilibrium dissociation constant (Kd) decreased in the summer and increased in the winter; whereas in the pinealectomy group, there was no significant change between the two different seasons. Conclusion: The expression of LH receptors in rat testicular cells had relationship with seasons; and the pineal body may be a top regulation site which induces the seasonal change of LH receptors.

关 键 词：实验研究 生殖 季节 松果林 黄体生成素受体 

分 类 号：R339.2[医药卫生—人体生理学]