咪唑克生对胰岛细胞的损伤效应及其机制  

The effect of idazoxan on pancreatic β-cells and its mechanism

作　　者：高洪伟[1] 

机构地区：[1]北京大学第三医院内分泌科,北京100083

出　　处：《中国药理学通报》2003年第2期174-176,共3页Chinese Pharmacological Bulletin

摘　　要：目的　观察咪唑克生 (idazoxan)对克隆胰岛素分泌细胞 (BRIN BD11细胞 )的损伤效应。方法　采用MTS还原法测定胰岛细胞活力 ,用流式细胞计 (FACS)进行细胞周期分析来测定咪唑克生对BRIN BD11细胞DNA完整性的影响。结果　①咪唑克生明显降低BRIN BD11细胞的活力 ,破坏DNA ,且具有时间和剂量依赖性。②genistein、5 HT1A受体拮抗剂NAN190和蛋白激酶G (PKG)抑制剂———KT5 82 3对咪唑克生引起的BRIN BD11细胞损伤没有保护作用。结论　咪唑克生对克隆胰岛素分泌细胞具有细胞毒效应 。AIM To investigate the effects of idazoxan on the viability and DNA integrity of clonal BRIN-BD11 cells. METHODS MTS reduction was used to check the viability of BRIN-BD11 cells. FACS cell cycle analysis was employed to determine the effects of idazoxan on DNA integrity of BRIN-BD11 cells. RESULTS ①Idazoxan markedly reduced the viability of BRIN-BD11 cells and resulted in DNA damage in time-and dose-dependent manner.②Genistein, 5-HT 1A antagonist NAN190 and PKG inhibitor-KT5823 had no protective effects against idazoxan-induced damage of BRIN-BD11 cells. CONCLUSION Idazoxan has cytotoxic effect and induces apoptosis of clonal pancreatic β-cells.

关 键 词：胰岛细胞 损伤效应 咪唑克生 BRIN-BD11 CELLS 细胞凋亡 

分 类 号：R972[医药卫生—药品]