切应力作用下联合培养的血管平滑肌细胞对内皮细胞PDGF-BmRNA表达的影响  

EFFECT OF CO-CULTURED VASCULAR SMOOTH MUSCLE CELLS ON PDGF-B mRNA EXPRESSION OF ENDOTHELIAL CELLS UNDER SHEAR STRESS

作　　者：李玉泉[1] 王栋[1] 张炎[1] 丛兴忠[1] 姜宗来[1] 

机构地区：[1]第二军医大学解剖学教研室生物医学工程研究所,上海200433

出　　处：《解剖学报》2003年第1期99-102,共4页Acta Anatomica Sinica

基　　金：国家自然科学基金资助项目 (3 0 0 70 19710 13 2 0 2 0 );国家重点基础研究发展规划 (973 )资助项目 (G2 0 0 0 0 5 69 0 5 )

摘　　要：目的　探讨切应力作用下联合培养的血管平滑肌细胞 (VSMCs)对内皮细胞 (ECs)PDGF BmRNA表达的影响 ,为预防血管移植物发生再狭窄提供实验资料。　方法　用原位杂交和图像分析等技术 ,以静态条件下单独培养的ECs和联合培养的ECs为两对照组 ,观察切应力作用下单独培养的ECs和与VSMCs联合培养ECs的PDGF BmRNA表达变化。　结果　静态条件下联合培养ECs的PDGF BmRNA表达水平比单独培养的ECs下降 ;切应力作用下 ,联合培养ECs的PDGF BmRNA的表达在切应力作用 1h左右有瞬时上升 ,6h后下降至低于联合培养条件下的静态水平 ,且瞬时上升的时间点比单独培养的ECs提前。　结论　切应力作用下 ,与VSMCs联合培养ECs的PDGF BmRNA表达水平下降 。Objective The effect of vascular smooth muscle cells (VSMCs) on the PDFG\|B mRNA expression of Co\|cultured endothelial cells (ECs) under shear stress had been studied in order to provide some experimental data for preventing the development of restenosis resulted from VSMCs proliferation in the vascular graft. Methods The level of PDGF\|B mRNA of ECs cultured alone and Co\|cultured with VSMCs exposed to shear stress were investigated by the methods of in situ hybridization and image analysis. Both controls of ECs cultured alone and Co\|cultured with VSMCs were maintained under static conditions. Results In static condition, the content of PDGF\|B mRNA expression of Co\|cultured ECs was lowered in comparison with ECs alone\|cultured. Under shear stress, PDGF\|B mRNA expression of Co\|cultured ECs was increased in transient manner at 1hour after the onset of shear stress and then returned to the level lower than the static level of Co\|cultured ECs at 6hours after being exposed to shear stress. The transient increasing time of PDGF\|B mRNA expression of Co\|cultured ECs reached a maximum earlier than that of ECs cultured alone.Conclusion Under shear stress, PDGF\|B mRNA expression of ECs Co\|cultured with VSMCs is lowered, which may be favor of inhibiting VSMCs proliferation.

关 键 词：血管平滑肌细胞 切应力 血小板源性生长因子 内皮细胞 

分 类 号：R318.01[医药卫生—生物医学工程]