一种新型呕吐动物模型——水貂  

A new vomiting animal model——mink

作　　者：岳旺[1] 张芳[1] 王蕾[1] 方选[1] 刘艳霞[1] 南胜[2] 

机构地区：[1]青岛大学医学院药理教研室,山东青岛266021 [2]日本国北海道医疗大学药理教研室

出　　处：《药学学报》2003年第2期89-91,共3页Acta Pharmaceutica Sinica

基　　金：国家教委归国留学人员基金资助项目 (教外司留[1995 ] 80 6号 );山东省卫生厅资助项目 ( 1999CA1CBA3)

摘　　要：目的　用水貂建立一种新型呕吐动物模型。方法　将水貂随机分组 ,给予 3种经典致呕剂顺铂、阿朴吗啡、硫酸铜及X射线全身照射 ,测定致吐反应和抗呕吐药的抗呕吐反应 ,最后取水貂肠管进行 5 HT免疫组化检测。结果　顺铂 ( 7 5mg·kg- 1 ,ip)、阿朴吗啡 ( 1 6mg·kg- 1 ,sc)和硫酸铜 ( 40mg·kg- 1 ,ig) 3种致呕剂均使水貂发生恶心、干呕和呕吐 ,顺铂引起水貂呕吐的阈值剂量为 5mg·kg- 1 。昂丹司琼和甲氧氯普胺可抑制顺铂和阿朴吗啡所致的干呕、呕吐。 5 HT免疫组化结果提示 ,肠道EC细胞 5 HT释放参与呕吐的发生机制。结论　水貂可作为一种新型呕吐模型 。Aim To establish a new, reliable vomiting model in minks. Methods Adult male minks ( Mustela vison ) were randomly divided into groups ( n =6). Cisplatin, apomorphine, copper sulfate and X radiation were used to establish vomiting model. Retching and vomiting were observed after the vomiting models were given anti vomiting agents. After the behavioral experiment, assay of 5 HT in the ileum was performed by immunohistologic method. Results Cisplatin 7 5 mg·kg -1 ip, apomorphine 1 6 mg·kg -1 sc and copper sulfate 40 mg·kg -1 ig were shown to evoke vomiting. Retching and vomiting were significantly inhibited in ondansetron and metoclopramide pretreated minks ( P <0 05, P <0 01). Conclusion As a new vomiting model, minks may be of great value in studying vomiting mechanism and screening new antiemetic drugs.

关 键 词：顺铂 昂丹司琼 阿朴吗啡 X射线 抗呕吐药 筛选 呕吐 动物模型 水貂 

分 类 号：R965.1[医药卫生—药理学] R975.4[医药卫生—药学]