皮内注药治疗疱疹后神经痛的机制  

Experimental study on the mechanism of the treatment of postherpetic neuralgia by intradermal drug injection

作　　者：章云海[1] 曾因明[1] 周震球[1] 李小明[1] 田月霞[1] 耿祝生[1] 赵琳[2] 张立丰[1] 张励才[1] 曹俊平[1] 

机构地区：[1]221002,徐州医学院,江苏省麻醉学重点实验室 [2]连云港市第一人民医院麻醉科

出　　处：《中华麻醉学杂志》2003年第2期115-117,共3页Chinese Journal of Anesthesiology

基　　金：江苏高校省级重点实验室第三批对外开放课题(K2093)

摘　　要：目的 在皮内注药治疗疱疹后神经痛(postherpetic neuralgia PHN)有效的基础上,通过动物实验探讨其疗效机制的神经学基础。方法 在家兔肩胛区皮内分别注射辣根过氧化物酶(HRP)和荧光素核黄(NY),用神经逆行追踪法研究皮内神经末梢到神经节神经细胞的上行轴浆流通道及初级神经元分布规律。结果 皮内注射HRP、NY分别在C4-T10脊神经节、颈、胸交感神经节、腹腔神经节、标记到神经细胞,脊神经节在C6-T8标记的细胞较多,交感神经节比脊神经节标记的细胞多,荧光标记神经细胞分布范围与HRP法基本一致或前后各多一个节段,而相同节段的标记神经细胞数明显比HRP标记的多。结论 动物实验从形态学上验证了皮内神经末梢到神经节神经细胞的上行轴浆流通道及初级神经元分布规律,皮内注药治疗PHN疗效可能与此通道有关。Objective To investigate the effectiveness of treating postherpetic neuralgia (PHN) by intradermal drug injection.Methods Ten rabbits of both sexes weighing 2.3-2.8 kg were anesthetized with intravenous urethane 1.5 g/kg. In group A (n = 5) 30% horseradish peroxidase(HRP) 500 μl and in group B (n = 5) 1%-2% fluorescent nuclear yellow (NY) 500μl were injected intradermally at 6-8 points along the both sides of spine in the scapula region. After 48-72 h the animals were sacrificed and C4 -T10 spinal ganglia, cervical and thoracic sympathetic ganglia and celiac ganglia were harvested for identification of labeled neurocytes. Results Labeled neurocytes were found in C4-T10 spinal ganglia, cervical and thoracic sympathetic ganglia and celiac ganglia. There were more labeled neurocytes in the C6-T8 spinal ganglia. There were more labeled neurocytes in the sympathetic ganglia than in the spinal ganglia. The distribution of fluorescent labeled neurocytes corresponded to neurocytes labeled by HRP method. At the same segment there were more fluorescent labeled neurocytes than neurocytes labeled by HRP. Conclusion There is an ascending axoplasma streaming channel from nerver ending to the neurocytes in the ganglion as shown by morphological study and the good therapeutic effect of intradermal drug injection in the treatment of PHN may be related to this channel.

关 键 词：皮内注药 治疗 疱疹后神经痛 带状疱疹 

分 类 号：R752.1[医药卫生—皮肤病学与性病学]