麻疹野病毒H1基因型在中国流行的分析  

An Analysis of Wild-type Measles Viruses of H1 Genotype Circulated in China

作　　者：许文波[1] 朱贞[1] 张珍英[2] 王同展[3] 蒋小泓[1] 王常银[3] 何维宽[4] 王建国[5] 李聪勇[2] 郑蕾[6] 凌华[7] 李平[8] 刘桂艳[9] 田宏[10] 周淑洁[4] 田疆[11] 王步安 

机构地区：[1]中国疾病预防控制中心病毒病预防控制所,北京100050 [2]河南省疾病预防控制中心,郑州450003 [3]山东省卫生防疫站,济南250014 [4]安徽省卫生防疫站,合肥230061 [5]上海市疾病预防控制中心,上海200336 [6]山西省疾病预防控制中心,太原030012 [7]重庆市疾病预防控制中心,重庆400042 [8]陕西省疾病预防控制中心,西安710054 [9]吉林省疾病预防控制中心,长春130021 [10]天津市卫生防病中心,天津300011 [11]辽宁省疾病预防控制中心,沈阳110005 [12]北京市地坛医院,北京100011

出　　处：《中国计划免疫》2003年第1期1-8,共8页Chinese Journal of Vaccines and Immunization

基　　金：世界卫生组织基金资助项目 (I8/1811/978)

摘　　要：为确定现阶段中国流行的麻疹野病毒本土基因型别 ,在 1995～ 2 0 0 2年用EB病毒转化的狨猴淋巴母细胞(B95a细胞 )从河南、山东、安徽、上海等 19个省 (自治区、直辖市 ,下同 )的麻疹爆发和散发病人的咽喉拭子或尿液标本中分离到麻疹病毒 15 6株。用逆转录 聚合酶链反应 (RT -PCR)从 15 6株麻疹病毒中扩增出核蛋白 (N)基因碳末端 4 5 0个核苷酸片段。通过对扩增产物的核苷酸序列测定和分析证明 ,15 4株为麻疹病毒H1基因型 ;2株属于A基因型 ,为沪191疫苗株。由此证实 ,H1基因型已在中国广泛流行 ,为中国麻疹病毒的优势基因型。H1是麻疹病毒型内变异最大的基因型。这 15 4株H1基因型麻疹病毒 ,除外Shanxi0 0 - 6和Hunan95 - 2 3株 ,可进一步划分为H1a和H1b两个亚组。H1a的 4 5 0个核苷酸和H1b的差异在 2 0 0 %～ 4 0 0 %。H1基因型的 4 5 0个核苷酸和A基因型代表株Edmonston的基因差异在 6 0 0 %～ 7 33% ;与H2基因型代表株China94 - 1的基因差异在 5 11%～7 5 6 % ;与其它 17个基因型代表株的最大差异在 11 5 6 %。在中国开展麻疹病毒监测和建立麻疹病毒毒株库及基因数据库 ,对加速麻疹控制及未来消除麻疹都十分必要。Genetic characterization of 156 measles viruses isolated from throat swabs or urine specimens of measles patients using B95a cell line in nineteen provinces of the People's Republic of China during 1995-2002 was studied.The sequence analysis of 450 nucleotides of COOH-terminal of nucleoprotein(N)genes of 156 isolates indicated that all of the Chinese viruses were members of H1 genotype except Hunan95-6 and Henan99-1,that was same with Chinese measles vaccine strain S191,and belonged to genotype A.These Chinese measles viruses of H1 genotype were more diverse as compared to the other genotypes and differed from genotype A(Edmonston)at 6.00%～7.33%,from genotype H2(China94-1)at 5.11%～7.56% and from other 17 genotypes as much as 11.56% in the COOH-terminal of the N gene.The Chinese measles virus H1 genotype is the most genetically divergent within single genotype described so far.The sequencing data suggest that H1 genotype of 154 Chinese measles viruses can further be divided into two small subgroups,the H1a and the H1b,except Shanxi100-6 and Hunan-23.The H1a subgroup differed from H1b by 2.00%～4.00% at the nucleotide level in the COOH-terminal of the N gene.It is helpful to develop measles virus surveillance and set up measles virus strain bank and virus gene bank to accelerate measles control and eliminate measles in future.

关 键 词：麻疹病毒 H1基因型 序列测定 麻疹野病毒 中国 

分 类 号：R373.11[医药卫生—病原生物学] R511.1[医药卫生—基础医学]