Effect of endothelin-1 on hepatic damage induced by endotoxin  

作　　者：刘宝华 肖南 陈惠孙 周继红 

机构地区：[1]Department of General Surgery,Daping Hospital,Research Institute of Surgery,Third Military Medical University

出　　处：《Chinese Journal of Traumatology》2000年第4期234-237,共4页中华创伤杂志（英文版）

摘　　要：To observe effect of endothelin-1 (ET-1) on hepatic damage induced by endotoxin. Methods: A total of 90 rats were randomly divided into control group (group C), endotoxin treated group (group LPS) and endotoxin plus ET-1 antibody treated group (group LEA). An observation was done on the changes of ET-1 concentration, and transcription and expression of ET-1 mRNA. Plasma glutamic pyruvic-transaminase enzyme (GPT), hepatic lactate dehydrogenase (LDH), adenosine triphosphate (ATP) and malondialdehyde (MDA) were also observed at 3, 6, 9, 12, 24 hours after saline, endotoxin (10 mg·kg -1 ) and ET-1 antibody (dalubine 1: 2*!000 , 2 ml·kg -1 ) administration. Results: The results indicated that the concentration of plasma and hepatic ET-1 and expression of ET-1 mRNA in liver significantly increased following endotoxemia. The hepatic ET-1 levels were inversely correlated with the ATP concentration, and positively related to the MDA concentration. ET-1 antibody could partially protect the liver against damage induced by endotoxin. Conclusions: These results suggest that endotoxin may, on transcription and translation level, lead to an increase of ET-1 in synthesis. ET-1 may contribute to hepatic damage during endotoxemia.To observe effect of endothelin-1 (ET-1) on hepatic damage induced by endotoxin. Methods: A total of 90 rats were randomly divided into control group (group C), endotoxin treated group (group LPS) and endotoxin plus ET-1 antibody treated group (group LEA). An observation was done on the changes of ET-1 concentration, and transcription and expression of ET-1 mRNA. Plasma glutamic pyruvic-transaminase enzyme (GPT), hepatic lactate dehydrogenase (LDH), adenosine triphosphate (ATP) and malondialdehyde (MDA) were also observed at 3, 6, 9, 12, 24 hours after saline, endotoxin (10 mg·kg -1 ) and ET-1 antibody (dalubine 1: 2*!000 , 2 ml·kg -1 ) administration. Results: The results indicated that the concentration of plasma and hepatic ET-1 and expression of ET-1 mRNA in liver significantly increased following endotoxemia. The hepatic ET-1 levels were inversely correlated with the ATP concentration, and positively related to the MDA concentration. ET-1 antibody could partially protect the liver against damage induced by endotoxin. Conclusions: These results suggest that endotoxin may, on transcription and translation level, lead to an increase of ET-1 in synthesis. ET-1 may contribute to hepatic damage during endotoxemia.

关 键 词：内毒素 肝损伤 内皮素-1 

分 类 号：R631[医药卫生—外科学] R575[医药卫生—临床医学]