Role of Fas/FasL genes in azoospermia or oligozoospermia induced by testosterone undecanoate in rhesus monkey  

Fas/FasL基因在11酸睾酮诱导恒河猴无精子症或少精子症中的作用(英文)

作　　者：周新昌[1] 魏鹏[1] 胡召元[1] 高飞[1] 邹如金[1] 刘以训 

机构地区：[1]中国科学院动物研究所生殖生物学国家重点实验室,北京中国100080

出　　处：《Acta Pharmacologica Sinica》2001年第11期1028-1032,共5页中国药理学报（英文版）

基　　金：Project supported by 973 and "Pandeng" Programs, National Natural Science Foundation of China (No 39870368, 39670285, 39970284);the Chinese Academy of Sciences (KJ982-JI-610)

摘　　要：AIM: To investigate role of apoptosis related genes Fas/ FasL in azoospermia or oligozoospermia induced by testosterone undecanoate. METHODS: TUNEL was used to detect the apoptotic signal of testicular cells. Immunohistochemistry and Western blot were used to quantitatively or qualitatively analyze the expression of these apoptosis-related proteins. RESULTS: After injection of testosterone undecanoate, both the apoptotic signal in germ cells and the expression of Fas/FasL in testis increased correlatively in a time-dependent manner, reaching a maximum level on day 30. CONCLUSION: Fas system may initiate and regulate the germ cell apoptosis induced by testosterone undecanoate.目的:研究凋亡相关基因FaS/FasL在11酸睾酮诱导无精子症或少精子症的作用。方法:TUNEL用于检测睾丸细胞的凋亡信号。免疫组化和Western blot用于对这些凋亡相关基因所表达的蛋白进行定量或定性分析。结果:11酸睾酮处理后,生精细胞的凋亡信号和Fas/FasL蛋白的表达呈现时间依赖性方式增强,至第30天达到最大值。结论:Fas系统可能参与启动和调节11酸睾酮诱导的生精细胞的凋亡。

关 键 词：Fas/FasL genes TESTOSTERONE APOPTOSIS macaca mulatta 

分 类 号：R698.2[医药卫生—泌尿科学]