PCR扩增技术在钩端螺旋体病早期诊断中的应用  

Rapid Detection of Leptospira Interrogans in Sera by Using PCR

作　　者：郭宗琪[1] 祝小平[1] 魏敏[1] 刘骊生[1] 黄自英[1] 蔺鸿[1] 欧阳兵[1] 刘伦光[1] 徐竹清[1] 杜娟[1] 

机构地区：[1]四川省疾病预防控制中心,成都610031

出　　处：《预防医学情报杂志》2003年第1期15-16,共2页Journal of Preventive Medicine Information

摘　　要：目的　研究钩体病患者血清中钩体DNA ,为防治该病提供科学依据。方法　应用PCR技术 ,测定患者血清钩体DNA和MAT法测血清中钩体抗体。结果　 4 0份患者早期血清中PCR法检出阳性8份 ,5 3份血清中MAT法检出阳性 11份。结论　PCR分子生物学技术应用钩体病早期诊断是一种快速敏感有效的方法 。Objective To study the DNA of Leptospira interrogans in patient's sera in order to provide a basis for scientific research. Methods DNA of Leptospira interrogans in patient's sera was detected by using PCR and antibody by MAT. Results Eight of forty patients by PCR and 11 of 53 by MAT were detected positive.Conclusions PCR could be a specific and sensitive molecular biological way for the rapid detection of Leptospirosis.

关 键 词：早期诊断 钩端螺旋体 聚合酶链反应 显微镜凝集实验 

分 类 号：R514.404[医药卫生—内科学]