同种异体肾移植后CD_4^+、CD_8^+、CD_(16)^+、CD_(19)^+、CD_(56)^+变化及临床意义  

Monitoring Peripheral Lymphocyte Phenotypes for Evaluation Immunosuppression Status in Renal Transplant Recipients

作　　者：李甦[1] 刘子栋[2] 王少梅[2] 王小平[2] 任万军[2] 朱彬[2] 高庆贞[2] 王丽[1] 许纯孝[3] 

机构地区：[1]山东省济南市第一人民医院,济南250013 [2]山东大学临床医学院济南市友谊肾脏病/血液净化中心,济南250013 [3]山东大学第二医院,济南250013

出　　处：《中国中西医结合肾病杂志》2003年第2期89-91,共3页Chinese Journal of Integrated Traditional and Western Nephrology

摘　　要：目的 :了解对比肾移植受者术后体内淋巴细胞免疫表型的变化情况及其临床意义。方法 :采用多种单克隆抗体及流式细胞仪 ,对肾移植受者术后的淋巴细胞免疫表型进行了化验监测 ,分析了淋巴细胞免疫表型变化与免疫用药及并发症的关系。结果 :移植受者淋巴细胞免疫表型阳性细胞数普遍偏低 (P <0 .0 5 ) ,肾移植后顺利恢复组的淋巴细胞免疫表型阳性细胞与健康对照组无差异 (P >0 .0 5 ) ,肾移植后并发症组受者CD+ 4 、CD+ 8阳性细胞数低于临床正常值 ,NK细胞及CD+ 19阳性细胞数减少 (P <0 .0 5 )。肾移植后急排组的NK细胞及CD+ 19阳性细胞数明显增加 ,CD+ 4 /CD+ 8比值偏高 (P <0 .0 5 )。结论 :淋巴细胞免疫表型可用于评价移植受者的免疫状态 ,临床上根据受者的化验结果综合评价受者的免疫抑制状态 ,并作为调整免疫抑制剂用量的参考指标。Objective:To monitor peripheral lymphocyte phenotypes and to evaluate the immunosuppression status by using FCM in reual fransplant recipient.Methods:The study included 36 stable recipients,15 recipients with complications such as uroleakage,bacterial infection and 9 recipients with actual rejection on regular immunosuppressive drugs.Results:Decreased precentage of peripheral lymphocyte phenotypes was found in group 1 and the other two groups of recipents have normal results.Conclusion:The precentages of activated lymphocyte evaluated by peripheral cell flow cytometry can be used as parameter to evaluate immunosupressive status and to optimize immunosuppressive regime in reual fransplant recipient.

关 键 词：同种异体肾移植 淋巴细胞免疫表型 流式细胞仪 

分 类 号：R392[医药卫生—免疫学]