糖基化终末产物对破骨细胞骨吸收功能的影响  

Effect of advanced glycosylation end products on bon e resorption by cultured osteoclasts

作　　者：蔡德鸿[1] 陈宏[1] 张桦[1] 郭楚霞[1] 王梅[1] 

机构地区：[1]第一军医大学附属珠江医院内分泌科,广州510282

出　　处：《中华内分泌代谢杂志》2003年第1期57-59,共3页Chinese Journal of Endocrinology and Metabolism

摘　　要：目的　观察糖基化终末产物 (AGEs)对破骨细胞骨吸收功能的影响。方法　用人血清白蛋白和葡萄糖恒温孵育制备人AGEs,采用甲苯胺蓝染色和图像分析评价AGE对破骨细胞形成骨吸收陷窝的影响。结果　低浓度AGEs(50～ 2 0 0 μg/ml)对骨吸收陷窝的面积和数目无明显影响 ;只有高浓度AGEs(50 0～ 1 0 0 0 μg/ml)才能促进骨吸收陷窝面积的扩大和数目增加 (P <0 .0 5)。Objective To observe th e effect of advanced glycosylation end products (AGEs) on bone resorption by cul tured osteoclasts. Methods Human serum albumin was incubated with glucose under constant temperature to prepare AGEs. The osteo clasts were isolated from human iliac spongy bone with collagenase Ⅱ and purifi ed on the basis of differences in attachment velocity. The area and number of re sorption pits in the adult bovine bone stained with toluidine blue were measured by the Leica Quantimet 500 system. Results Sma ller dose of AGEs induced little changes in the resorption pits, but larger dose of AGEs significantly enhanced the formation of bone resorption pits (P<0.05). Conclusion AGEs seem to a ccelerate bone resorption by cultured osteoclasts.

关 键 词：高级糖基化终产物 破骨细胞 骨质吸收 糖尿病 骨质疏松 

分 类 号：R587.1[医药卫生—内分泌]