ICP-AES法测定人血清中的硒  

Determination of Se in Serum by ICP-AES

作　　者：方奕文[1] 贾丽[1] 余林[1] 宋一兵[1] 孙长勇[1] 

机构地区：[1]汕头大学化学系分析测试中心,广东汕头515063

出　　处：《光谱学与光谱分析》2003年第1期73-74,共2页Spectroscopy and Spectral Analysis

基　　金：汕头大学工业催化学科"2 11工程"建设项目资助

摘　　要：采用消化法预处理血清样品 ,并用ICP AES法测定样品中的Se ,同时 ,探索Fe的存在对测定的干扰情况以及干扰的消除方法。结果表明 ,Se的检测限为 0 0 0 76μg·mL- 1 ,加标回收率为 91 6 % ,1 0次测定的RSD(n =1 0 )为 2 1 4 7% ;在样品中加入KOH ,可以大大降低Fe对Se测定的干扰。The serum sample solution was prepared by using acid digestion and the Se concentration in it was determined by ICP-AES. At the same time, the interference from Fe and the interference-free method were studied. The results showed that the recovery of standard addition was 91.6%, the relative standard deviation(n=10)was 2.147% and when KOH was added to the sample solution the interference was reduced greatly. The method is efficient, accurate and easy to operate.

关 键 词：ICP-AES法 规定 血清 硒 人体 临床诊断 电感偶合等离子体-原子发射光谱 

分 类 号：R446.11[医药卫生—诊断学]