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机构地区:[1]中国科学技术大学化学与材料科学学院,合肥230026
出 处:《Chinese Journal of Chemical Physics》2003年第1期75-78,共4页化学物理学报(英文)
基 金:美国NorlandProductsInc资助项目
摘 要:利用凝胶排阻层析 火焰原子吸收光谱法研究了Ag+ 在照相明胶中分子量的分布情况 .研究表明 ,利用葡聚糖凝胶G 75可有效的将明胶中的γ、β、α及小分子蛋白L组分分开 .发现Ag+ 完全分布于分子量较小的α大分子蛋白组分 .γ组分、β组分中均不存在Ag+ .The distribution of Ag + in gelatin with respect to molecular weight was studied using gel exclusion chromatography―flame atomic a bsorption spectroscopy. Firstly, several gels used for isolating gelatin protein components were studied and the result indicated that dextran G 75 was favoura ble for the present study. The γ, β, α and other lower molecular weight compo nents of gelatin could be isolated from each other by means of gel G 75. The re sults demonstrated that the Ag + in gelatin was combined only with the α prote in component and not with the γ or the β components. [WT5HZ
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