毛细管电泳用于胞嘧啶脱氨反应动力学研究  

Dynamic Study on Cytosine Deamination by Capillary Electrophoresis

作　　者：王平[1] 任吉存[1] 

机构地区：[1]湖南师范大学化学化工学院,长沙410081

出　　处：《分析科学学报》2003年第1期13-16,共4页Journal of Analytical Science

基　　金：国家自然科学基金 (No.2 9975 0 0 8);湖南省自然科学基金 (No.0 0 JJY2 0 1 3 )

摘　　要：DNA中的胞嘧啶在化学致变剂作用下会发生脱氨反应 ,被认为是导致基因损伤的重要原因之一。本文采用毛细管电泳测定胞嘧啶和尿嘧啶 ,并将此种新方法用于过氧化氢存在下胞嘧啶脱氨反应的动力学研究 ,结果显示该反应对过氧化氢和胞嘧啶均为一级反应 ,反应活化能为 70 .3 5k J·mol- 1,明显低于自发脱氨反应 。Cytosine deamination has been considered to be one of the key reasons for DNA damage. In the paper, we first developed a sensitive and highly selective method to determine contents of cytosine and uracil based on capillary electrophoresis with a UV absorbance detector. Then, we used this method to investigate the cytosine deamination to uracil in the presence of hydrogen peroxide. Data obtained showed that the reaction orders for cytosine and hydrogen peroxide were both one order and the activation energy was 70.35 kJ·mol -1, lower apparently than that of spontaneous reaction.

关 键 词：毛细管电泳 胞嘧啶 脱氨反应 反应动力学 毛细管电泳 

分 类 号：Q526.1[生物学—生物化学]