有机小分子硫堇与DNA相互作用的研究  

Study of the Interaction between Thionine and DNA

作　　者：冶保献[1] 刘灵芳[1] 齐小花[1] 王翠红[1] 

机构地区：[1]郑州大学化学系,郑州450052

出　　处：《分析科学学报》2003年第1期51-53,共3页Journal of Analytical Science

基　　金：河南省杰出青年基金 (No.990 2 4 )

摘　　要：用光谱电化学和电化学技术研究了硫堇和硫堇与 DNA相互作用的电化学和光谱电化学性质 ,得出了硫堇作为有机小分子能插入 DNA双螺旋结构内部的结论 ,求得了对应的动力学参数。DNA的加入量与硫堇峰电流的降低呈一次线性关系 ,可望用于This paper reports our recent research about the electrochemical character of thionine and the interaction between thionine and DNA by electrochemistry and spectroelectrochemistry technologies. The results show that thionine can insert into the inner of the DNA bi-helix structure. The kinetics parameters, the number of transferred electrons, standard potential E 0' and reaction rate constant are obtained by spectroelectrochemistry with results of 1.033, -0.209 V and 2.51×10 -3 s -1, respectively. Moreover, the thionine peak currents are decreased linearly with increased DNA concentration.

关 键 词：有机小分子 DNA 硫堇 光谱电化学 相互作用 

分 类 号：Q523[生物学—生物化学]